Effect of Baicalein on Matrix Metalloproteinases and Durability of Resin-Dentin Bonding
Effect of Baicalein on Matrix Metalloproteinases and Durability of Resin-Dentin Bonding
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DOI：
10.2341/17-097-l
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发表时间：
2018-07-01
影响因子：
2.2
通讯作者：
Li, Y.
中科院分区：
文献类型：
--
作者：
Li, J.;Chen, B.;Li, Y.
目的：为了提高树脂牙本质的粘合质量，本研究使用黄芩素作为蚀刻和冲洗粘合剂中的预处理剂，以评估其对基质金属蛋白酶（MMP）和粘合剂耐久性的影响。方法：作为一种 MMP 抑制剂和潜在的胶原交联剂，黄芩素被用作蚀刻和冲洗粘合剂系统中的预处理剂。通过傅里叶变换红外光谱评价转化程度。采用EnzChek明胶酶/胶原酶测定试剂盒检测不同浓度黄芩素（0.1、0.5、2.5、5.0μg/mL）对牙本质粉的MMP抑制作用。在体外粘接过程中，健全的第三磨牙的平坦牙本质表面在酸蚀后用2.5μg/mL黄芩素进行预处理；此步骤之后是继续粘合过程和树脂复合材料的构建。树脂-牙本质棒制备后，立即或在人工唾液中储存三至六个月后，分别通过微拉伸测试、立体显微镜和场发射扫描电子显微镜评估粘合强度、失效模式和界面纳米渗漏。数据采用双向方差分析和Tukey检验(α=0.05)进行分析。结果:黄芩素在0-5.0μg/mL浓度范围内不影响粘合剂的转化率。然而，它抑制牙本质粘结明胶酶和胶原酶的活性，特别是在2.5 μg/mL浓度下，同时在体外立即和老化后均能有效提高微拉伸粘结强度并减少纳米渗漏。结论：黄芩素作为酸蚀冲洗粘结剂体系中的预处理剂具有抗MMP功能，可有效提高树脂牙本质粘结耐久性，在临床粘结过程中具有潜在价值。
Objective: In an attempt to increase resindentin bonding quality, this study used baicalein as a preconditioner in an etch-and-rinse adhesive to evaluate its effect on matrix metalloproteinases (MMPs) and adhesive durability.Methods: As a MMP inhibitor and potential collagen cross-linking agent, baicalein was used as a preconditioner in an etch-and-rinse adhesive system. The degree of conversion was evaluated by Fourier-transform infrared spectroscopy. EnzChek gelatinase/collagenase assay kits were then used to detect the MMP inhibitory effect of different concentrations of baicalein (0.1, 0.5, 2.5, and 5.0 mu g/mL) on dentin powders. During in vitro bonding procedures, flat dentin surfaces on sound third molars were preconditioned with 2.5 mu g/mL baicalein after being acid-etched; this step was followed by continuation of adhesive processes and build-up of resin composite. After resin-dentin stick preparation, bonding strength, failure mode, and interface nanoleakage were respectively evaluated via microtensile testing, stereomicroscopy, and field emission scanning electron microscopy either immediately or after storage in artificial saliva for three or six months. Data were analyzed by two-way analysis of variance and Tukey test (alpha=0.05).Results: Baicalein at a concentration of 0-5.0 mu g/mL did not influence the conversion of adhesives. However, it inhibited the activities of dentin-bond gelatinase and collagenase, especially at a concentration of 2.5 mu g/mL, while effectively increasing microtensile bonding strength and decreasing nanoleakage in vitro, both immediately and after aging.Conclusions: Baicalein used as preconditioner in an etch-and-rinse adhesive system has an anti-MMP function and effectively improves resindentin bonding durability in vitro, which has potential value in clinical bonding procedures.