Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus.
Transcriptomic and metabolomic analyses for providing insights into the influence of polylysine synthetase on the metabolism of Streptomyces albulus.
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DOI：
10.1186/s12934-022-01953-8
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发表时间：
2022-10-28
影响因子：
6.4
通讯作者：
Qin, Jiayang
中科院分区：
文献类型：
--
作者：
Lian, Congcong;Zhang, Min;Mao, Jiaqi;Liu, Yuanyu;Wang, Xiuwen;Kong, Linghui;Yao, Qingshou;Qin, Jiayang
ε-聚赖氨酸（ε-PL）是白色链霉菌的主要次级代谢产物，在食品工业中有着广泛的应用。多聚赖氨酸合成酶（Polylysine synthetase，Pls）是ε-PL生物合成途径中的最后一个酶。我们以前的研究表明，Pls在S. albulus CICC 11022菌株的发酵产物中ε-PL的含量较高。本研究初步构建了Pls基因敲除菌株。本研究采用基因组学、转录组学和代谢组学相结合的方法，研究了Pls基因的高表达和敲除对S.白色。Pls的高表达导致598个显著差异表达基因（DEG）和425个已知的差异代谢物，而Pls的失活导致868个显著差异表达基因和374个已知的差异代谢物。与Pls表达负相关的基因有8个，与Pls表达正相关的基因有35个。阐明了Pls的高表达和失活对ε-PL生物合成途径的影响机制。在Pls高表达菌株中鉴定出12种代谢产物，产量降低30%，但在Pls敲除菌株中产量增加30%。这些结果说明了磷脂对S.白色。本研究为利用代谢工程提高ε-PL的生产能力或开发S.白色。在线版本包含补充材料，可通过10.1186/s12934-022-01953-8获得。
ε-poly-l-lysine (ε-PL) is the main secondary metabolite of Streptomyces albulus, and it is widely used in the food industry. Polylysine synthetase (Pls) is the last enzyme in the ε-PL biosynthetic pathway. Our previous study revealed that Pls overexpressed in S. albulus CICC11022 result in the efficient production of ε-PL. In this study, a Pls gene knockout strain was initially constructed. Then, genomic, transcriptomic and metabolomic approaches were integrated to study the effects of the high expression and knockout of Pls on the gene expression and metabolite synthesis of S. albulus. The high expression of Pls resulted in 598 significantly differentially expressed genes (DEGs) and 425 known differential metabolites, whereas the inactivation of Pls resulted in 868 significant DEGs and 374 known differential metabolites. The expressions of 8 and 35 genes were negatively and positively associated with the Pls expression, respectively. Subsequently, the influence mechanism of the high expression and inactivation of Pls on the ε-PL biosynthetic pathway was elucidated. Twelve metabolites with 30% decreased yield in the high-expression strain of Pls but 30% increased production in the Pls knockout strain were identified. These results demonstrate the influence of Pls on the metabolism of S. albulus. The present work can provide the theoretical basis for improving the production capacity of ε-PL by means of metabolic engineering or developing bioactive substances derived from S. albulus. The online version contains supplementary material available at 10.1186/s12934-022-01953-8.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.5
作者：
Czech L;Hermann L;Stöveken N;Richter AA;Höppner A;Smits SHJ;Heider J;Bremer E
通讯作者：
Bremer E
影响因子：
6.1
作者：
Liu, Jia-Ning;Chang, Sen-Lin;Zhao, Qing-Sheng
通讯作者：
Zhao, Qing-Sheng
影响因子：
0.8
作者：
Bhattacharyya, K;Mazumdar, SK;Palmer, RA
通讯作者：
Palmer, RA
氨基酸和dapA基因过表达对产色链霉菌菌株α-聚-L-赖氨酸生产的影响
影响因子：
2.6
作者：
Li, Wenchao;Lv, Junge;Jia, Shiru
通讯作者：
Jia, Shiru