Transduction of full-length TAT fusion proteins into mammalian cells:: TAT-p27Kip1 induces cell migration

Transduction of full-length TAT fusion proteins into mammalian cells:: TAT-p27Kip1 induces cell migration
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DOI:
10.1038/4042
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发表时间:
1998-12-01
期刊:
影响因子:
82.9
通讯作者:
Dowdy, SF
Dowdy, SF
中科院分区:
医学1区
文献类型:
--
作者:
Nagahara, H;Vocero-Akbani, AM;Dowdy, SF

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图1 pTAT-HA载体和纯化方案。a,pTAT-HA表达载体。B,此处使用达特融合蛋白。KK和N分别表示Cdk结合和Mg 2+配位结构域中的点突变9,10。c,变性和达特融合蛋白纯化方案。a B c pTAT-HA
Fig. 1 The pTAT-HA vector and purification protocol. a, pTAT-HA expression vector. b, TAT fusion proteins used here. KK and N indicate point mutations in the Cdk binding and Mg2+ coordinating domains, respectively9, 10. c, Denaturation and TAT fusion protein purification protocol. a b c pTAT-HA