Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.

Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.
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质膜:通过使用带正电荷的珠子快速分离和暴露细胞质表面。

DOI:
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发表时间:
1977
期刊:
影响因子:
56.9
通讯作者:
D. Branton
D. Branton
中科院分区:
综合性期刊1区
文献类型:
--
作者:
B. Jacobson;D. Branton

文献摘要

被引文献

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将红细胞离子连接到直径为30微米的聚赖氨酸包被的珠上。细胞的结合如此牢固,以至于附着细胞的裂解或破坏留下被质膜覆盖的珠,其细胞质表面暴露并可用于进一步分析。
Erythrocytes were ionically attached to polylysine-coated beads 30 micrometers in diameter. The binding of the cells was so tenacious that lysis or disruption of the attached cells left the beads covered by plasma membranes whose cytoplasmic surface was exposed and accessible for further analysis.