The process of displacing the single-stranded DNA-binding protein from single-stranded DNA by RecO and RecR proteins.
The process of displacing the single-stranded DNA-binding protein from single-stranded DNA by RecO and RecR proteins.
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DOI：
10.1093/nar/gkm1004
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发表时间：
2008-01
影响因子：
14.9
通讯作者：
Mikawa T
中科院分区：
文献类型：
--
作者：
Inoue J;Honda M;Ikawa S;Shibata T;Mikawa T
DNA 损伤产生的单链 (ss) DNA 区域立即被 ssDNA 结合蛋白 (SSB) 覆盖。 RecF 途径蛋白促进 SSB 从 ssDNA 中置换，使 RecA 蛋白在 ssDNA 区域形成蛋白丝，从而促进重组 DNA 修复过程。在本研究中，我们使用纯化的嗜热栖热菌 RecF 途径蛋白研究了 ssDNA 中 SSB 置换的机制。迄今为止，RecO 和 RecR 被认为充当 RecOR 复合体。然而，我们的结果表明 RecO 和 RecR 具有不同的功能。我们发现RecR同时结合RecF和RecO，并且RecO结合RecR、SSB和ssDNA。电子显微镜研究表明 SSB 被 RecO 从 ssDNA 中取代。此外，下拉分析表明，置换的 SSB 仍然通过与 RecO-ssDNA 复合物中的 RecO 相互作用而间接附着在 ssDNA 上。在 SSB 和 RecO 同时存在的情况下，RecA 的 ssDNA 依赖性 ATP 酶活性受到抑制，但通过添加 RecR 可以恢复。有趣的是，RecR 与 RecO 的相互作用影响了 RecO 的 ssDNA 结合特性。这些结果提出了 RecF 途径蛋白从 ssDNA 中 SSB 置换的模型。
The regions of single-stranded (ss) DNA that result from DNA damage are immediately coated by the ssDNA-binding protein (SSB). RecF pathway proteins facilitate the displacement of SSB from ssDNA, allowing the RecA protein to form protein filaments on the ssDNA region, which facilitates the process of recombinational DNA repair. In this study, we examined the mechanism of SSB displacement from ssDNA using purified Thermus thermophilus RecF pathway proteins. To date, RecO and RecR are thought to act as the RecOR complex. However, our results indicate that RecO and RecR have distinct functions. We found that RecR binds both RecF and RecO, and that RecO binds RecR, SSB and ssDNA. The electron microscopic studies indicated that SSB is displaced from ssDNA by RecO. In addition, pull-down assays indicated that the displaced SSB still remains indirectly attached to ssDNA through its interaction with RecO in the RecO-ssDNA complex. In the presence of both SSB and RecO, the ssDNA-dependent ATPase activity of RecA was inhibited, but was restored by the addition of RecR. Interestingly, the interaction of RecR with RecO affected the ssDNA-binding properties of RecO. These results suggest a model of SSB displacement from the ssDNA by RecF pathway proteins.
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DOI：
10.1073/pnas.252633399
发表时间：
2002-11-26
影响因子：
11.1
作者：
Kantake, N;Madiraju, MVVM;Kowalczykowski, C
通讯作者：
Kowalczykowski, C
影响因子：
5.6
作者：
LUISIDELUCA, C;KOLODNER, R
通讯作者：
KOLODNER, R
影响因子：
2.9
作者：
KURAMITSU, S;HIROMI, K;KAGAMIYAMA, H
通讯作者：
KAGAMIYAMA, H
DOI：
10.1073/pnas.94.13.6652
发表时间：
1997-06-24
影响因子：
11.1
作者：
Raghunathan, S;Ricard, CS;Waksman, G
通讯作者：
Waksman, G
影响因子：
11.4
作者：
Lee, BI;Kim, KH;Suh, SW
通讯作者：
Suh, SW