The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain.
The invasion process of the entomopathogenic fungus Ophiocordyceps sinensis into the larvae of ghost moths (Thitarodes xiaojinensis) using a GFP-labeled strain.
复制标题
使用 GFP 标记菌株研究昆虫病原真菌 Ophiocordyceps sinensis 侵入鬼蛾 (Thitarodes xiaojinensis) 幼虫的过程
DOI：
10.3389/fmicb.2022.974323
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
冬虫夏草是一种著名的名贵中药材，是冬虫夏草寄生鬼蛾幼虫后形成的。冬虫夏草自然感染率较低，限制了冬虫夏草的大规模人工栽培，且其入侵过程尚不清楚。为了研究中华蝗进入鬼蛾时的时空调控，我们构建了稳定表达绿色荧光蛋白（GFP）的中华蝗转化体。用高浓度的GFP标记的中华圆线虫接种小金丝蜱幼虫，观察到中华圆线虫分生孢子在2天内即可粘附在寄主角质层上，在4天内萌发穿透钉，并在6天内在寄主血腔中产生芽生孢子。入侵部位重建的三维 (3D) 结构表明，穿透钉直接从中华 O. sinensis 分生孢子在与幼虫角质层的连接部位萌发。沿着寄主上角质层分化的附着胞或菌丝并不是中华圆线虫入侵鬼蛾所必需的。总体而言，阐明了中华圆线虫入侵宿主的具体过程，为昆虫病原真菌的入侵过程提供了新的视角。
Chinese cordyceps is a well-known and valuable traditional Chinese medicine that forms after Ophiocordyceps sinensis parasitizes ghost moth larvae. The low natural infection rate of O. sinensis limits large-scale artificial cultivation of Chinese cordyceps, and the invasion process is unclear. To investigate the temporal and spatial regulation when O. sinensis enters ghost moths, we constructed an O. sinensis transformant that stably expresses green fluorescent protein (GFP). Inoculating Thitarodes xiaojinensis larvae with a high concentration of GFP-labeled O. sinensis, we observed that O. sinensis conidia could adhere to the host cuticle within 2 days, germinate penetration pegs within 4 days, and produce blastospores in the host hemocoel within 6 days. The reconstructed three-dimensional (3D) structures of the invasion sites showed that penetration pegs germinated directly from O. sinensis conidia at the joining site with the larval cuticle. Differentiated appressoria or hyphae along the host epicuticle are not required for O. sinensis to invade ghost moths. Overall, the specific invasion process of O. sinensis into its host is clarified, and we provided a new perspective on the invasion process of entomopathogenic fungi.
影响因子：
5.2
作者：
Zhang P;Cui S;Ren X;Kang S;Wei F;Ma S;Liu B
通讯作者：
Liu B