A simple method to distinguish between simian immunodeficiency virus isolates by restriction analysis of PCR products.
A simple method to distinguish between simian immunodeficiency virus isolates by restriction analysis of PCR products.
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一种通过 PCR 产物的限制性分析来区分猿猴免疫缺陷病毒分离株的简单方法。
DOI：
10.1089/dna.1993.12.617
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发表时间：
1993
影响因子：
3.1
通讯作者：
Chuang,RY
中科院分区：
文献类型：
--
作者：
Blackbourn,DJ;Sutjipto,S;Doi,RH;Chuang,RY
本文报道了一种简单的方法来区分实验感染恒河猴的猴免疫缺陷病毒（SIV）分离株。外周血单核细胞（PBMC）从感染了最初从残尾猕猴分离的SIVStM的恒河猴或从感染了来自短尾白眉猴的SIVSM的恒河猴制备。将PBMC与CEM × 174细胞共培养，用聚合酶链反应（PCR）扩增SIV囊膜（env）基因。PCR产物的限制酶消化分析使SIVStM和SIVSM能够彼此区分，并与SIVMAC的分子克隆SIVMAC 239区分，SIVMAC 239最初从感染的恒河猴分离。此外，当SIVSM和SIVStM被引入到同一动物中时，从该恒河猴的共培养细胞扩增的PCR产物的限制性酶分析表明，该动物被重叠感染。
A simple method to distinguish between simian immunodeficiency virus (SIV) isolates of experimentally infected rhesus macaques is reported. Peripheral blood mononuclear cells (PBMC) were prepared from a rhesus macaque infected with SIVStMisolated originally from a stump-tailed macaque, or from a rhesus monkey infected with SIVSMfrom a sooty mangabey monkey. PBMC were cocultivated with CEM × 174 cells and a region of the SIV envelope (env) gene was amplified by the polymerase chain reaction (PCR) from cDNA of infected cocultivation cells. Restriction enzyme digestion analysis of the PCR products enabled SIVStMand SIVSMto be differentiated from each other, and from a molecular clone of SIVMAC, SIVMAC239, originally isolated from an infected rhesus macaque. Furthermore, when SIVSMand SIVStMwere introduced into the same animal, restriction enzyme analysis of the PCR product amplified from cocultivation cells of this rhesus macaque suggested that the animal was superinfected.