STIMULATION OF PROTEIN-SYNTHESIS BY LYSINE ANALOGS IN LYSINE-DEPRIVED EHRLICH ASCITES TUMOR-CELLS
STIMULATION OF PROTEIN-SYNTHESIS BY LYSINE ANALOGS IN LYSINE-DEPRIVED EHRLICH ASCITES TUMOR-CELLS
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DOI：
10.1016/0167-4889(84)90093-4
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
CLEMENS, MJ
中科院分区：
文献类型：
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作者：
AUSTIN, SA;CLEMENS, MJ
研究了氨基酸饥饿调节哺乳动物细胞中蛋白质合成起始的机制。检查了一系列赖氨酸类似物刺激培养物中缺乏赖氨酸的小鼠艾氏腹水肿瘤细胞中蛋白质合成的能力。在这些测试的类似物中，只有那些在细胞内裂解为赖氨酸的类似物能够将蛋白质合成恢复到完全喂养的细胞中所见的水平。与琼脂糖共价连接的赖氨酸不会刺激翻译。将缺乏赖氨酸的细胞与类似物赖氨酸对硝基苯胺孵育 5 分钟后，细胞提取物中的赖氨酸浓度恢复到补给细胞提取物中的浓度，并且在 5-10 分钟内最大程度地刺激蛋白质合成。在此期间，培养基中赖氨酸的浓度没有增加。可能是细胞内氨基酸池的大小而不是细胞外氨基酸池的大小调节了氨基酸剥夺期间蛋白质合成的速率。
The mechanism by which amino acid starvation regulates the initiation of protein synthesis in mammalian cells was investigated. The ability of a range of lysine analogs to stimulate protein synthesis in lysine-deprived mouse Ehrlich ascites tumor cells in culture was examined. Of those analogs tested, only those which are cleaved to lysine intracellularly are capable of restoring protein synthesis to the level seen in fully fed cells. Lysine which is covalently linked to agarose does not stimulate translation. After 5 min incubation of lysine-deprived cells with the analog lysine p-nitroanilide, the lysine concentration in cell extracts is restored to that found in extracts from fed cells, and protein synthesis is maximally stimulated within 5-10 min. During this period of time there is no increase in the concentration of lysine in the medium. It is probably the size of the intracellular rather than the extracellular amino acid pool which regulates the rate of protein synthesis during amino acid deprivation.