Functional channels in mature E. coli colonies
Functional channels in mature E. coli colonies
复制标题
成熟大肠杆菌菌落中的功能通道
DOI：
10.1101/851428
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
G. McConnell
中科院分区：
文献类型：
--
作者：
L. Rooney;W. Amos;P. Hoskisson;G. McConnell
生物膜在医学、工业和自然环境中非常重要，但其结构在多个空间尺度上基本上未被探索。我们已经研究了成熟的大肠杆菌大菌落生物膜的结构的Mesolens，一个光学系统，它独特地允许单个细菌和超过一百立方毫米的生物膜环境的同时成像。我们的主要发现是在E.大肠杆菌生物膜。这些通道具有一种特殊的结构，并在殖民地完全机械分解后进行改革。我们提出的证据表明，通道运输颗粒和功能，以帮助吸收营养物质。这些通道可能为将分散剂或抗微生物药物递送到生物膜提供新的途径，最终降低其对公共卫生和工业的影响。
Biofilms are important in medicine, industry and the natural environment, however their structure is largely unexplored across multiple spatial scales. We have studied the architecture of mature Escherichia coli macro-colony biofilms by means of the Mesolens, an optical system which uniquely allows simultaneous imaging of individual bacteria and over one hundred cubic millimetres of its biofilm milieu. Our chief finding is the presence of intra-colony channels on the order of 10 μm in diameter in E. coli biofilms. These channels have a characteristic structure and reform after total mechanical disaggregation of the colony. We present evidence that the channels transport particles and function to assist the absorption of nutrients. These channels potentially offer a new route for the delivery of dispersal agents or antimicrobial drugs to biofilms, ultimately lowering their impact on public health and industry.