REGULATION OF NISIN BIOSYNTHESIS AND IMMUNITY IN LACTOCOCCUS-LACTIS 6F3
REGULATION OF NISIN BIOSYNTHESIS AND IMMUNITY IN LACTOCOCCUS-LACTIS 6F3
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DOI：
10.1128/aem.60.3.814-825.1994
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发表时间：
1994-03-01
影响因子：
4.4
通讯作者：
ENTIAN, KD
中科院分区：
文献类型：
--
作者：
ENGELKE, G;GUTOWSKIECKEL, Z;ENTIAN, KD
乳酸菌乳球菌6F3的生物合成基因以结构基因nisA开始，然后是nisB、nisT和nisC基因，它们可能参与了前肽的化学修饰和分泌（G. Engelke， Z. Gutowski-Eckel， M. Hammelmann， K.D. Entian, Appl）。环绕。中华微生物学杂志，1998,28(3):357 - 357。邻近的5kb下游区域的亚克隆发现了参与nisin生物合成的其他基因。nisI基因编码一种脂蛋白，在转化为对nisI敏感的乳酸乳杆菌MG1614后，可提高免疫力。它后面是基因nisP，编码一种枯草菌素样丝氨酸蛋白酶，可能参与分泌的前导肽的加工。在nisP的3'端，鉴定出nisR和nisK基因，编码一个调节蛋白和一个组氨酸激酶，与双组分调控系统的OmpR/ envz样亚群成员有明显的相似性。nisR和nisK的氨基酸序列与SpaR和SpaK有明显的相似性，SpaR和SpaK最近被确定为枯草素生物合成的反应调节因子和相应的组氨酸激酶。通过分别针对nisin前肽和NisB蛋白的抗体，我们可以证明nisin的生物合成受其结构和生物合成基因的表达调控。Prenisin的表达开始于指数生长阶段，比NisB蛋白的表达早约30分钟。这两种蛋白在固定生长阶段表达到最大值。
The biosynthetic genes of the nisin-producing strain Lactococcus lactis 6F3 are organized in an operon-like structure starting with the structural gene nisA followed by the genes nisB, nisT, and nisC, which are probably involved in chemical modification and secretion of the prepeptide (G. Engelke, Z. Gutowski-Eckel, M. Hammelmann, and K.D. Entian, Appl. Environ. Microbiol. 58:3730-3743, 1992). Subcloning of an adjacent 5-kb downstream region revealed additional genes involved in nisin biosynthesis. The gene nisI, which encodes a lipoprotein, causes increased immunity after its transformation into nisin-sensitive L. lactis MG1614. It is followed by the gene nisP, coding for a subtilisin-like serine protease possibly involved in processing of the secreted leader peptide. Adjacent to the 3' end of nisP the genes nisR and nisK were identified, coding for a regulatory protein and a histidine kinase, showing marked similarities to members of the OmpR/EnvZ-like subgroup of two-component regulatory systems. The deduced amino acid sequences of nisR and nisK exhibit marked similarities to SpaR and SpaK, which were recently identified as the response regulator and the corresponding histidine kinase of subtilin biosynthesis. By using antibodies directed against the nisin prepeptide and the NisB protein, respectively, we could show that nisin biosynthesis is regulated by the expression of its structural and biosynthetic genes. Prenisin expression starts in the exponential growth phase and precedes that of the NisB protein by approximately 30 min. Both proteins are expressed to a maximum in the stationary growth phase.