Serology of Neisseria gonorrhoeae: W-antigen serogrouping by coagglutination and protein I serotyping by enzyme-linked immunosorbent assay both detect protein I antigens
Serology of Neisseria gonorrhoeae: W-antigen serogrouping by coagglutination and protein I serotyping by enzyme-linked immunosorbent assay both detect protein I antigens
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淋病奈瑟氏菌血清学：通过共凝集进行的 W 抗原血清分型和通过酶联免疫吸附测定进行的蛋白 I 血清分型均检测蛋白 I 抗原
DOI：
10.1128/iai.35.1.229-239.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
T. Buchanan
中科院分区：
文献类型：
--
作者：
E. Sandstrom;J. Knapp;T. Buchanan
用凝集试验(COA)对224株菌株进行分型，用蛋白I酶联免疫吸附试验(ELISA)进行分型。在这些菌株中，61株来自新加坡、菲律宾和丹麦的播散性淋球菌感染患者，21株来自盆腔炎患者，115株来自无并发症的淋球菌感染患者。27株为实验室参考菌株。在患者菌株中，102株属于COA血清组WI型，100株蛋白I血清型为1、2或3型的菌株全部属于这一组。61例播散性淋病患者的淋球菌菌株大多在这一组(COAWI，53或87%；蛋白I血清型1、2或3，51或84%)。所有46株属于蛋白I血清型4至7的菌株也都是COA血清组Wii。蛋白I血清型8和9型占197株患者菌株中的49株(25%)。其中28株COA血清群为Wii，20株为Will，1株为Wii和Wii。CoA-W血清型和蛋白I-EL ISA均可检测到淋球菌外膜蛋白I分子上的抗原。蛋白I血清分型使用未煮过的生物，通常可以识别更多的可变和表面暴露的抗原决定因素。相反，使用煮过的生物体的COA W血清组除了识别可变的蛋白I抗原决定簇外，还可能识别暴露较少的共享抗原决定簇。这两种方法都可能被证明对进一步研究淋病的流行病学和发病机制有用。
A total of 224 strains were serogrouped by coagglutination (COA) and serotyped by protein I enzyme-linked immunosorbent assay (ELISA). Of these strains, 61 were from patients with disseminated gonococcal infection, 21 were from patients with pelvic inflammatory disease, and 115 were from patients with uncomplicated gonococcal infection in Singapore, the Philippines, and Denmark. Twenty-seven were laboratory reference strains. Of the patient strains, 102 belonged to COA serogroup WI, and all of the 100 strains that typed with protein I serotypes 1, 2, or 3 were in this group. Most of the strains of gonococci from the 61 patients with disseminated gonococcal infection were within this group (COA WI, 53 or 87%; protein I serotypes 1, 2, or 3, 51 or 84%). All 46 strains that were protein I serotypes 4 through 7 were also COA serogroup WII. Protein I serotypes 8 and 9 accounted for 49 (25%) of the 197 patient strains. Twenty-eight of these strains typed as COA serogroup WII, 20 typed as serogroup WIII, and 1 typed as serogroups WII and WIII. COA W serogrouping and protein I ELISA both appeared to detect antigens on the protein I molecule of the outer membrane of Neisseria gonorrhoeae. Protein I serotyping, which uses unboiled organisms, may generally recognize more variable and surface-exposed antigenic determinants. In contrast, COA W serogrouping, which uses boiled organisms, may recognize less exposed shared antigenic determinants in addition to variable protein I antigenic determinants. Both methods may prove useful for further studies of the epidemiology and pathogenesis of gonorrhea.