MONOCLONAL-ANTIBODY TO HLA-A3
MONOCLONAL-ANTIBODY TO HLA-A3
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DOI：
10.1089/hyb.1.1982.1.87
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
CRESSWELL, P
中科院分区：
文献类型：
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作者：
BERGER, AE;DAVIS, JE;CRESSWELL, P
单克隆抗体 GAP A3 可检测 HLA 同种异体特异性 A3。单克隆抗体的反应性与 A3 抗原的存在完全一致，A3 抗原是通过常规同种异体抗血清在一组 59 个来自具有明确 HLA 抗原的个体的细胞上定义的。在一个家庭中，八个兄弟姐妹中的三个继承了父系 A3 抗原，与 GAP A3 的反应性与 HLA-A3 分离。在还原条件下，GAP A3 在 SDS 聚丙烯酰胺凝胶上从 HLA-A3 阳性而非 HLA-A3 阴性 B 淋巴母细胞系中沉淀出适当的 44,000 道尔顿和 12,000 道尔顿条带。因此，根据血清学、家族和生化标准，GAP A3 定义了 HLA-A3 的同种异体特异性。
The monoclonal antibody GAP A3 detects the HLA allospecificity A3. Reactivity of the monoclonal was in exact concordance with the presence of the A3 antigen as defined by conventional alloantisera on a panel of 59 cells from individuals with well-characterized HLA antigens. Reactivity with GAP A3 segregated with HLA-A3 in a family where three of eight siblings inherited the paternal A3 antigen. GAP A3 precipitated appropriate 44,000- and 12,000-dalton bands on SDS-polyacrylamide gels under reducing conditions from an HLA-A3-positive, but not an HLA-A3-negative B lymphoblastoid cell line. Thus, by serological, familial, and biochemical criteria, GAP A3 defines the allospecificity HLA-A3.