Characterization of a small plasmid carrying the carbapenem resistance gene blaOXA-72 from community-acquired Acinetobacter baumannii sequence type 880 in China
Characterization of a small plasmid carrying the carbapenem resistance gene blaOXA-72 from community-acquired Acinetobacter baumannii sequence type 880 in China
复制标题
中国社区获得性鲍曼不动杆菌880型序列中携带碳青霉烯类抗生素抗性基因blaOXA-72的小质粒的鉴定
DOI：
10.2147/idr.s202803
复制
发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Xie, Xinyou
中科院分区：
文献类型：
--
作者：
Jia, Huiqiong;Sun, Qingyang;Xie, Xinyou
背景：鲍曼不动杆菌已成为与医院和社区获得性感染相关的重要病原体。社区获得的A.鲍氏肺炎的特征是暴发性病程和高死亡率。在这项研究中，我们报告了一个社区获得性碳青霉烯类耐药的A。方法：采用PCR方法扩增携带bla（OXA-72）基因的鲍曼不动杆菌。鲍曼不动杆菌分离株是从一名诊断为社区获得性肺炎、败血性休克和呼吸衰竭的男性患者中回收的。采用微量肉汤稀释法测定其最低抑菌浓度。使用长读MinION和短读Illumina平台进行全基因组测序，以充分表征A.鲍曼不动杆菌A52。通过我们最近更新的BacWGSTdb服务器进一步阐明了密切相关的分离株的计算机多位点序列分型和基因组流行病学分析。结果：该分离株对美罗培南耐药，对其他几种抗菌药物敏感。全基因组测序和生物信息学分析表明，该A.鲍曼不动杆菌分离株属于罕见的散发性克隆序列类型880，bla（OXA-72）基因位于8，493-bp质粒pA 52-OXA-72上。该质粒与各种不动杆菌属中的不同OXA-72编码质粒（大小范围：8，771 - 12，056 bp）仅表现出部分相似性。结论：本研究描述了首例暴发性碳青霉烯类耐药的社区获得性A。中国罕见的散发性克隆引起的鲍曼不动杆菌肺炎。有必要进行充分的监测，以监测A。鲍曼不动杆菌作为一种社区病原体。
Background: Acinetobacter baumannii has emerged as an important pathogen associated with hospital- and community-acquired infections. Community-acquired A. baumannii pneumonia is characterized by a fulminant course and high mortality rates. In this study, we report the identification of a community-acquired carbapenem-resistant A. baumannii strain carrying the bla(OXA-72) gene.Methods: This A. baumannii isolate was recovered from a male patient diagnosed with community-acquired pneumonia, septic shock, and respiratory failure. Antimicrobial susceptibility testing were performed and the minimum inhibitory concentrations were determined by the broth microdilution method. Whole-genome sequencing was performed using both long-read MinION and short-read Illumina platforms to fully characterize the bla(OXA-72)-carrying plasmid of the A. baumannii A52. The in silico multilocus sequence typing and genomic epidemiological analysis of the closely related isolates were further elucidated by our recently updated BacWGSTdb server.Results: The isolate was resistant to meropenem and remained susceptible to several other antimicrobial agents. Whole-genome sequencing and bioinformatics analysis indicated that this A. baumannii isolate belonged to the rare sporadic clone sequence type 880 and the bla(OXA-72) gene was located on the 8,493-bp plasmid pA52-OXA-72. This plasmid exhibited only partial similarity to different OXA-72-encoding plasmids (size range: 8,771-12,056 bp) in various Acinetobacter spp. recovered from patients and other reservoirs in different countries.Conclusion: This study described the first case of fulminant carbapenem-resistant community-acquired A. baumannii pneumonia caused by a rare sporadic clone in China. Adequate surveillance is warranted to monitor the emergence of A. baumannii as a community pathogen.