Neurofilament protein is phosphorylated in the squid giant axon.
Neurofilament protein is phosphorylated in the squid giant axon.
复制标题
神经丝蛋白在鱿鱼巨型轴突中磷酸化。
DOI：
10.1083/jcb.78.2.r23
复制
发表时间：
1978-08
影响因子：
7.8
通讯作者：
Lasek, R J
中科院分区：
文献类型：
--
作者：
Pant, H C;Shecket, G;Gainer, H;Lasek, R J
我们观察到鱿鱼轴浆中神经丝蛋白的磷酸化。磷酸化通过轴浆与 [gamma-32P]ATP 孵育过程中蛋白质的 32P 标记来证明。当标记的蛋白质通过 SDS 聚丙烯酰胺凝胶电泳 (SDS-PAGE) 分离时，两条带（2.0 x 10(5) 道尔顿和大于 4 x 10(5) 道尔顿）包含大部分 32P。 2.0 x 10(5)-道尔顿磷酸化多肽在 SDS-PAGE 上与鱿鱼神经丝蛋白的亚基之一共迁移。两种主要的磷酸化多肽在不连续蔗糖梯度离心和 Sepharose 4B 上的凝胶过滤层析中与神经丝共分级。蛋白质-磷酸键的行为类似于磷酸酯，并且在蛋白质的酸水解产物中鉴定出标记的磷酸丝氨酸。讨论了这种现象在不同物种中的普遍性及其可能的生理意义。
We have observed the phosphorylation of neurofilament protein from squid axoplasm. Phosphorylation is demonstrated by 32P labeling of protein during incubation of axoplasm with [gamma-32P]ATP. When the labeled proteins are separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), two bands, at 2.0 x 10(5) daltons and greater than 4 x 10(5) daltons, contain the bulk of the 32P. The 2.0 x 10(5)-dalton phosphorylated polypeptide comigrates on SDS-PAGE with one of the subunits of squid neurofilament protein. Both major phosphorylated polypeptides co-fractionate with neurofilaments in discontinuous sucrose gradient centrifugation and on gel filtration chromatography on Sepharose 4B. The protein-phosphate bond behaves like a phospho-ester, and labeled phospho-serine is identified in an acid hydrolysate of the protein. The generality of this phenomenon in various species and its possible physiological significance are discussed.