Optimal collection and storage conditions for catecholamine measurements in human plasma and urine.
Optimal collection and storage conditions for catecholamine measurements in human plasma and urine.
复制标题
人血浆和尿液中儿茶酚胺测量的最佳收集和储存条件。
作者：
Gironi;A. Seghieri;Niccolat M;Mammini P Simultaneous;Binder;Sr Sivorinovsky
儿茶酚胺（CA）浓度测定方法的改进，导致越来越多地使用这些化合物作为筛选患者和长期临床试验中的标记物。由于相关的后勤问题，我们研究了未解决的样品制备和血浆和尿液样品储存的最佳条件问题。结果表明：采血后1 h内离心；没有必要使用冷冻离心机。一旦准备好血浆，CA在20℃下稳定1天，在4℃下稳定2天，在-20℃下稳定1个月（或添加谷胱甘肽后稳定6个月），在-70℃下稳定1年。CA在4℃下稳定1个月，在未保存的尿液中稳定4个月，在EDTA和代谢亚硫酸钠保存的尿液中稳定4个月。在酸化尿液中，CA在4℃和-20℃下经过1年后几乎没有变化。
Improvements in methodologies for measuring concentrations of catecholamines (CA) have led to an increasing use of these compounds as markers in the screening of patients and in long-term clinical trials. Because of the associated logistical problems, we have investigated the unresolved question of optimal conditions for sample preparation and for storage of plasma and urine samples. Results show that blood should be centrifuged within 1 h after collection; the use of a refrigerated centrifuge is not necessary. Once plasma is prepared, CA are stable for 1 day at 20 degrees C, 2 days at 4 degrees C, 1 month at -20 degrees C (or 6 months with added glutathione), and up to 1 year at -70 degrees C. CA are stable at 4 degrees C for 1 month in unpreserved urine and for 4 months in urine preserved with EDTA and sodium metabisulfite. In acidified urine, CA were nearly unchanged after 1 year at 4 and -20 degrees C.