Protocol for Differential Biopanning of P. falciparum Phage Display cDNA Library to Identify Parasite Targets of Protective Antibodies.
Protocol for Differential Biopanning of P. falciparum Phage Display cDNA Library to Identify Parasite Targets of Protective Antibodies.
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恶性疟原虫噬菌体展示 cDNA 文库的差异生物淘选方案，以识别保护性抗体的寄生虫靶标。
DOI：
10.1007/978-1-0716-2189-9_26
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Raj,DipakKumar
中科院分区：
文献类型：
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作者：
Zuromski,Jenna;Kurtis,Jonathan;Raj,DipakKumar
疟疾仍然是一个重大的全球健康负担，每年造成数十万儿童死亡（世卫组织，世界疟疾报告。WHO，日内瓦，2019）。尽管经过几十年的努力，仍然没有广泛有效的疫苗。寄生虫噬菌体展示文库的差异筛选是鉴定由抗性个体而非易感个体表达的人抗体的靶标的有前景的方法（Raj等人，Nature，582，104-108，2020; Science，344，871-877，2014）。我们的全蛋白质组差异筛选（WPDS）的方法包括积极的选择，以捕捉噬菌体结合抗疟疾个体表达的抗体，然后通过负选择，以消除噬菌体结合疟疾易感个体表达的抗体，并扩增差异识别的克隆。
Malaria remains a significant global health burden, killing hundreds of thousands of children annually (WHO, The world malaria report. WHO, Geneva, 2019). Despite decades of effort, no broadly effective vaccine exists. Differential screening of parasite phage display libraries is a promising approach to identify the targets of human antibodies expressed by resistant but not by susceptible individuals (Raj et al., Nature, 582, 104–108, 2020; Science, 344, 871–877, 2014). Our whole proteome differential screening (WPDS) approach consists of positive selection to capture phage that bind antibodies expressed by malaria-resistant individuals, followed by negative selection to remove phage that bind antibodies expressed by malaria-susceptible individuals, and amplification of differentially recognized clones.