Leishmania RNA viruses in Leishmania of the Viannia subgenus
Leishmania RNA viruses in Leishmania of the Viannia subgenus
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DOI：
10.4269/ajtmh.1996.54.425
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发表时间：
1996-04-01
影响因子：
3.3
通讯作者：
Saravia, N
中科院分区：
文献类型：
--
作者：
Salinas, G;Zamora, M;Saravia, N
对起源于哥伦比亚东南部和西南部地区的69株利什曼原虫的核型分析表明，巴西利什曼原虫的四个株以及世界卫生组织参考菌株L.guyanensis IWHI/BR/78/M5313中的RNA约为5.3kb。从4例患者的皮肤和粘膜病变中分离的该参考毒株和巴西钩端螺旋体中的RNA元件与古扬氏钩端螺旋体CUMC-1-1A(LRV1-1)株中存在的RNA病毒的cDNA制备的RNA探针杂交。这些毒株还含有一个80kD的蛋白，与针对LRV1-1外壳蛋白(衣壳)的重组片段制备的多克隆抗体发生反应。此外，另一株哥伦比亚巴西乳杆菌被发现含有大约3.5kb的RNA，该RNA不与LRV1-1探针杂交。与含有5.3kb RNA的菌株相比，该菌株的总裂解物不含与衣壳蛋白片段的抗血清反应的物质。迄今为止发现的所有含有LRV1相关病毒的利什马尼亚病毒都起源于亚马逊河流域。核型分析和生物学特性分析表明，从高转移株5313获得的17个克隆都保留了约5.3kb的RNA，且没有分离出具有转移特性的病毒。与LRV1相关的病毒在亚马逊河流域流行的巴西钩端螺旋体和古扬氏钩端螺旋体中的分布有限，使这些成分成为潜在的流行病学标记。
Karyotype analysis of 69 strains of Leishmania belonging to three species of the Viannia subgenus originating from the southeastern and southwestern regions of Colombia revealed approximately 5.3-kb RNAs in four strains of L. braziliensis and also in the World Health Organization reference strain L. guyanensis IWHI/BR/78/M5313. The RNA element in this reference strain and in L. braziliensis strains isolated from cutaneous and mucosal lesions of four patients hybridized with RNA probes prepared from cDNA of the RNA virus present in L. guyanensis strain CUMC-1-1A (LRV1-1). These strains also contained an 80-kD protein that reacted with polyclonal antibody prepared against a recombinant fragment of the coat (capsid) protein of LRV1-1. In addition, another Colombian strain of L. braziliensis was found to contain an approximately 3.5-kb RNA that did not hybridize with LRV1-1 probes. Contrasting with the strains containing the 5.3-kb RNA, a total lysate of this strain did not contain material reactive with antiserum to the capsid protein fragment. All Leishmania containing LRV1-related viruses identified to date have originated in the Amazon River basin. Karyotype analyses and biological characterization of 17 clones obtained from the highly metastatic L. guyanensis strain 5313 revealed retention of the approximately 5.3 kb RNA in all clones and no segregation of the virus with the metastatic trait. The restricted distribution of LRV1-related viruses among some strains of L. braziliensis and L. guyanensis circulating in the Amazon River basin makes these elements potential epidemiologic markers.