Expression of DJ-1 proteins in placentas from women with severe preeclampsia
Expression of DJ-1 proteins in placentas from women with severe preeclampsia
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DOI：
10.1016/j.ejogrb.2012.12.024
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发表时间：
2013-05-01
影响因子：
2.6
通讯作者：
Park, Seung-Hwa
中科院分区：
文献类型：
--
作者：
Kwon, Han-Sung;Hwang, Han-Sung;Park, Seung-Hwa
目的：DJ-1是近年来发现的一种新的缺氧诱导细胞反应的介质。本研究的目的是确定DJ-1的表达在正常和先兆子痫preeclamptic pregnances.Study设计的妇女胎盘的差异：DJ-1 mRNA表达定量逆转录聚合酶链反应（RT-PCR）。结果：研究组DJ-1 mRNA的表达水平明显高于对照组（P < 0. 05），而对照组DJ-1 mRNA的表达水平明显低于研究组（P < 0. 05）。免疫反应性是特别高的子痫前期胎盘的合体滋养细胞相比，对照group.Conclusion：DJ-1蛋白的过度表达在重度PE患者的胎盘被认为是一个原因或代偿机制，在应对缺氧，这一发现可能有助于更好地了解子痫前期的病理生理。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Objective: Lately, DJ-1 has been identified as a novel mediator of hypoxia-induced cellular responses. The aim of this study was to determine the difference of DJ-1 expression in the placentas of women with normal and preeclamptic pregnancies.Study design: DJ-1 mRNA expression was quantified by quantitative reverse transcription polymerase chain reaction (RT-PCR). Also, immnoperoxidase and immunofluorescent staining for DJ-1 was performed on 11 normal and 12 preeclamptic placental tissues.Results: The expression of mRNA of DJ-1 was significantly higher in the study group than in the control group. The immunoreactivity was especially higher in the syncytiotrophoblast of preeclamptic placentas compared to the control group.Conclusion: Overexpression of the DJ-1 protein in the placentas of severe PE patients is thought to be a causative or compensatory mechanism in response to hypoxia, and this finding might contribute to a better understanding of the pathophysiology of preeclampsia. (C) 2013 Elsevier Ireland Ltd. All rights reserved.