The prebiotic effect of Anoectochilus formosanus and its consequences on bone health
The prebiotic effect of Anoectochilus formosanus and its consequences on bone health
复制标题
DOI：
10.1017/s0007114512003777
复制
发表时间：
2013-05-28
影响因子：
3.6
通讯作者：
Lin, Wen-Chuan
中科院分区：
文献类型：
--
作者：
Yang, Li-Chan;Wu, Jin-Bin;Lin, Wen-Chuan
本研究评估台湾金线莲标准化水提物（SAEAF）的益生元效应及其对去卵巢（OVX）大鼠骨质疏松症的影响。去卵巢大鼠随机分为5组，分别灌胃水、SAEAF（200和400 mg/kg/d）和菊粉（400 mg/kg/d）12周。假手术组灌胃水。SAEAF治疗增加了OVX大鼠粪便中双歧杆菌的数量。钙平衡实验结果表明，SAEAF提高了钙的表观吸收和存留。SAEAF治疗12周后处死OVX大鼠。SAEAF降低了盲肠pH值，增加了盲肠壁重量、盲肠粘膜钙结合蛋白-D9 k mRNA表达、盲肠中游离钙浓度和SCFA水平。骨矿物质含量，密度和生物力学强度在OVX大鼠低于假手术组，但这些骨丢失被阻止SAEAF管理。显微断层扫描显示，SAEAF治疗组大鼠的骨小梁体积高于OVX组大鼠。这些结果表明，SAEAF防止骨质流失与卵巢激素缺乏症的大鼠。
The present study evaluated the prebiotic effect of a standardised aqueous extract of Anoectochilus formosanus (SAEAF) and its effects on osteoporosis in ovariectomised (OVX) rats. The OVX rats were randomly divided into five groups and orally treated with water, SAEAF (200 and 400 mg/kg daily) and inulin (400 mg/kg daily) for 12 weeks. The sham group was orally treated with water. The SAEAF treatment enhanced the number of faecal bifidobacteria in OVX rats. The results of a Ca-balance experiment showed that SAEAF increased apparent Ca absorption and retention. The OVX rats were killed after SAEAF treatment lasting 12 weeks. The SAEAF decreased the caecal pH values and increased the caecal wall weight, caecal mucosa calbindin-D9k mRNA expression, free-Ca concentration and levels of SCFA in the caecum. The mineral content, density and biomechanical strength of bones were lower in OVX rats than the sham group, but these bone losses were prevented by SAEAF administration. Microtomography scanning showed that the SAEAF-treated rats had higher trabecular bone volume than the OVX rats. These results suggest that SAEAF prevented bone loss associated with ovarian hormone deficiency in the rats.