A nano-needle/microtubule composite gliding on a kinesin-coated surface for target molecule transport.
A nano-needle/microtubule composite gliding on a kinesin-coated surface for target molecule transport.
复制标题
纳米针/微管复合材料在驱动蛋白涂层表面上滑动，用于目标分子运输。
作者：
M. Tarhan;R. Yokokawa;C. Bottier;D. Collard;H. Fujita
通过使用运动蛋白已经实现了微/纳米运输的另一种方法。驱动蛋白涂层表面上的微管有可能充当纳米运输系统。当微管用作载体时，货物或货物连接物附着在微管表面上。这种货物附着物可以显着影响驱动蛋白的运动。为了解决分子附着在微管表面造成的困难，货物装载和运输机制应分开。在这项工作中，我们建议使用微机械针作为货物载体，然后可以在微管上运输。由于针功能化和运输机制的分离，针的功能化可以在不对微管结构产生任何影响的情况下进行，从而显着增加了可能的货物类型。我们使用简单有效的方法大量制造了硅针，并表明微管-针复合材料的运输不会影响驱动蛋白的活性。
An alternative method of micro/nano-transport has been achieved by using motor proteins. Microtubules on a kinesin-coated surface have potential to act as a nano-transport system. When microtubules are used as carriers, either cargo or cargo linkers are attached on the microtubule surface. Such cargo attachments can significantly affect kinesin motion. To deal with the difficulty caused by molecular attachment to the microtubule surface, the cargo loading and transport mechanism should be separated. In this work, we propose to use micromachined needles as cargo carriers which then can be transported on microtubules. Because of the separation of needle functionalization and transport mechanism, functionalization of the needles can proceed without any effect on the microtubule structure, significantly increasing the possible types of cargo. We have fabricated silicon needles in mass numbers using a simple and effective method and have shown that the microtubule-needle composites are transported without affecting the kinesin activity.
微管相关蛋白 (MAP) 的纯化和测定。
影响因子：
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作者：
Sloboda,RD;Rosenbaum,JL
通讯作者：
Rosenbaum,JL