Modified wick method using Weck-Cel sponges for collection of human rectal secretions and analysis of mucosal HIV antibody.
Modified wick method using Weck-Cel sponges for collection of human rectal secretions and analysis of mucosal HIV antibody.
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DOI：
10.1097/00126334-200008010-00001
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发表时间：
2000-08
期刊：
影响因子：
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通讯作者：
P. Kozlowski;R. Lynch;Rosalyn R. Patterson;Susan Cu-Uvin;T. Flanigan;M. Neutra
中科院分区：
文献类型：
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作者：
P. Kozlowski;R. Lynch;Rosalyn R. Patterson;Susan Cu-Uvin;T. Flanigan;M. Neutra
检查了Weck-Cel海绵作为非创伤性收集人类直肠分泌物的吸收材料的适用性。在体外对海绵进行测试，并通过定量酶联免疫吸附测定（ELISA）确定海绵在用补充洗涤剂的缓冲液处理后能够释放100%吸收的白蛋白和所有免疫球蛋白亚型。随后比较了用干海绵（DS）或用盐水润湿软化的海绵（MS）收集的正常妇女直肠分泌物中的蛋白质组成。DS分泌物显示血液和间质液来源的白蛋白、免疫球蛋白G（IgG）和单体伊加污染的证据。MS分泌物似乎更准确地代表了局部粘膜分泌物，因为它们含有可忽略不计的血液，总伊加中分泌型伊加的百分比更大，与相应的血清相比，白蛋白/IgG比值更低，IgG亚类分布的变化更大。用湿海绵收集的8例HIV感染妇女的直肠分泌物中，ELISA分别可显示抗HIV IgG、IgM、伊加和分泌成分的抗体。数据显示，如果预先湿润，Weck-Cel海绵可用于非创伤性收集直肠液，并获得有关直肠粘膜表面免疫球蛋白和特异性抗体浓度的定量信息。
Weck-Cel sponges were examined for suitability as an absorbent material for nontraumatic collection of rectal secretions in humans. Sponges were tested in vitro and determined by quantitative enzyme-linked immunosorbent assay (ELISA) to be capable of releasing 100% of absorbed albumin and all immunoglobulin subtypes after treatment with detergent-supplemented buffer. Protein composition in rectal secretions collected from normal women with dry sponges (DS) or with sponges previously softened by moistening with saline (MS) was subsequently compared. DS secretions showed evidence of contamination with blood and interstitial fluid-derived albumin, immunoglobulin G (IgG), and monomeric IgA. MS secretions appeared to represent local mucosal secretions more accurately because they contained negligible blood, a greater percentage of secretory IgA within the total IgA, and both lower albumin/IgG ratios and more dramatic alterations in IgG subclass distribution compared with corresponding serum. Anti-HIV IgG, IgM, IgA, and antibodies with secretory component could be demonstrated by ELISA in rectal secretions collected with moist sponges from 8 of 8, 1 of 8, 5 of 8, and 3 of 8 HIV-infected women, respectively. The data show that Weck-Cel sponges, if premoistened, can be used to collect rectal fluids nontraumatically and to obtain quantitative information about concentrations of immunoglobulins and specific antibodies on rectal mucosal surfaces.