Expression of IL-33 in chronic lesional skin of canine atopic dermatitis
Expression of IL-33 in chronic lesional skin of canine atopic dermatitis
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DOI：
10.1111/vde.12531
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发表时间：
2018-06-01
影响因子：
1.4
通讯作者：
Maeda, Sadatoshi
中科院分区：
文献类型：
--
作者：
Asahina, Ryota;Nishida, Hidetaka;Maeda, Sadatoshi
背景-在人类中，白细胞介素（IL）-33在增强过敏性皮肤炎症中起关键作用。然而，目前还不清楚是否IL-33参与犬特应性皮炎（cAD）的发病机制。目的-检查IL-33在慢性病变皮肤cAD.Animals的表达-8只狗自发性cAD和5个健康dogs.Methods -IL-33在慢性病变皮肤cAD的转录进行了定量逆转录PCR。用与犬IL-33有交叉反应的抗人IL-33单克隆抗体检测IL-33的表达。角质形成细胞是cAD慢性病变皮肤中IL-33产生的主要细胞来源。结论和临床意义-结果表明IL-33参与cAD慢性病变皮肤。
Background - In humans, interleukin (IL)-33 plays a critical role in the enhancement of allergic skin inflammation. However, it currently remains unclear whether IL-33 is involved in the pathogenesis of canine atopic dermatitis (cAD).Objectives - To examine the expression of IL-33 in chronic lesional skin of cAD.Animals - Eight dogs with spontaneous cAD and five healthy dogs were used.Methods - The transcription of il-33 in chronic lesional skin of cAD was quantified by quantitative reverse transcription PCR. The expression of IL-33 was evaluated immunohistochemically using an anti-human IL-33 monoclonal antibody with cross-reactivity to canine IL-33.Results - The transcription levels of il-33 in chronic lesional skin of cAD were significantly higher than those in normal skin of healthy dogs. Keratinocytes were a major cellular source of IL-33 production in chronic lesional skin of cAD.Conclusions and clinical importance - The results indicate that IL-33 is involved in chronic lesional skin of cAD.