Intrafamilial, Preferentially Mother-to-Child and Intraspousal, Helicobacter pylori Infection in Japan Determined by Mutilocus Sequence Typing and Random Amplified Polymorphic DNA Fingerprinting
Intrafamilial, Preferentially Mother-to-Child and Intraspousal, Helicobacter pylori Infection in Japan Determined by Mutilocus Sequence Typing and Random Amplified Polymorphic DNA Fingerprinting
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DOI：
10.1111/hel.12217
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发表时间：
2015-10-01
期刊：
影响因子：
4.4
通讯作者：
Shiraishi, Tsukasa
中科院分区：
文献类型：
--
作者：
Yokota, Shin-ichi;Konno, Mutsuko;Shiraishi, Tsukasa
背景资料：幽门螺杆菌的感染途径主要是家庭内感染，尤其是在发达国家。为了确定传播途径，我们研究了多位点序列分型（MLST）是否有助于分析家庭内感染。材料与方法：采用MLST和随机扩增多态性DNA（RAPD）指纹图谱两种基因分型方法，对35例日本儿科患者及其家庭成员的菌株进行了克隆关系分析。MLST检测结果显示，35例患儿中有9例（25.7%）的等位基因与父亲和母亲均匹配，25例（60.0%）的等位基因与母亲匹配。pylori作为高度可重复的方法。家庭内，尤其是母婴和兄弟姐妹感染是主要的传播途径。据认为，在日本家庭中，约有四分之一的人会发生配偶内感染。
Background: The infection route of Helicobacter pylori has been recognized to be mainly intrafamilial, preferentially mother-to-child, especially in developed countries. To determine the transmission route, we examined whether multilocus sequence typing (MLST) was useful for analysis of intrafamilial infection. The possibility of intraspousal infection was also evaluated.Materials and Methods: Clonal relationships between strains derived from 35 index Japanese pediatric patients, and their family members were analyzed by two genetic typing procedures, MLST and random amplified polymorphic DNA (RAPD) fingerprinting.Results: Mostly coincident results were obtained by MLST and RAPD. By MLST, the allele of loci in the isolates mostly matched between the index child and both the father and mother for 9 (25.7%) of the 35 patients, between the index child and the mother for 25 (60.0%) of the 35 patients.Conclusions: MLST is useful for analyzing the infection route of H. pylori as a highly reproducible method. Intrafamilial, especially mother-to-children and sibling, infection is the dominant transmission route. Intraspousal infection is also thought to occur in about a quarter in the Japanese families.