Isolation and characterization of Phragmites australis (L.) rhizosphere bacteria from contaminated site for bioremediation of colored distillery effluent
Isolation and characterization of Phragmites australis (L.) rhizosphere bacteria from contaminated site for bioremediation of colored distillery effluent
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DOI：
10.1016/j.ecoleng.2006.02.008
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发表时间：
2006-10-02
影响因子：
3.8
通讯作者：
Rai, Vibhuti
中科院分区：
文献类型：
--
作者：
Chaturvedi, Sonal;Chandra, Ram;Rai, Vibhuti
基于甘蔗糖蜜的厌氧消化的酿酒厂流出物是深棕色的，这是由于高浓度的类黑精（氨基羰基聚合物）、酚类物质、重金属和硫酸盐，其即使在长时间曝气后也不会改变。因此，流出物是不雅观的并且引起环境问题。芦苇相关的根际细菌群落表现出脱色造纸废水，但细菌种类的性质和细节是未知的。根据16 SrRNA序列分析，从根际土壤中鉴定出15种可培养细菌，它们分别是：氧化烃微杆菌（AJ 880397）、木糖氧化无色杆菌（AJ 880764）、枯草芽孢杆菌（AJ 880760）、枯草芽孢杆菌（AJ 880760）、木聚糖杆菌（AJ 880764）。巨大菌（AJ 880767）、B. anthracis（AJ880766）; licheniformis（AJ 880762）、地衣芽孢杆菌A.木糖氧化菌（AJ 880763）、无色杆菌属（AJ 880396）、B.苏云金芽孢杆菌（AJ 868359）、苏云金芽孢杆菌（B. licheniformis（AJ880758），B.枯草杆菌（AJ 880761）和表皮葡萄球菌（AJ 880759）、混合假单胞菌（AJ 887999）、粪产碱菌（AJ 880765）B.蜡状芽孢杆菌AJ 853737来自根际下层。所有15个分离物在作为唯一碳源的污水补充培养基上生长，导致酿酒厂污染物水平和它们的颜色降低75.5%。同时，生物需氧量（BOD）、化学需氧量（COD）、苯酚、硫酸盐、重金属也有所减少。本研究的实际应用是对深色造纸废水的脱色。(c)2006 Elsevier B. V.保留所有权利。
The sugarcane molasses-based anaerobically digested distillery effluent is dark brown due to high concentration of melanoidin (amino carbonyl polymer), phenolics, heavy metals, and sulfate which do not alter even after long extended aeration. Thus, effluent is unsightly and poses an environmental concern. The Phragmites australis associated rhizosphere bacterial communities showed decolorization of distillery effluent but the nature and details of bacterial species are unknown. Fifteen culturable bacterial sp. were identified based on the 16S rRNA sequencing from the rhizosphere soil; they are Microbacterium hydrocarbonoxydans (AJ880397), Achromobacter xylosoxidans (AJ880764), Bacillus subtilis (AJ880760), B. megaterium (AJ880767), B. anthracis (AJ880766) from upper zone; B. licheniformis (AJ880762), A. xylosoxidans (AJ880763), Achromobacter sp. (AJ880396), B. thuringiensis (AJ868359), B. licheniformis (AJ880758), B. subtilis (AJ880761) from middle zone and Staphylococcus epidermidis (AJ880759), Pseudomonas migulae (AJ887999), Alcaligens faecalis (AJ880765) B. cereus (AJ853737) from lower zone of rhizosphere. All the fifteen isolates grew on effluent supplemented medium as a sole carbon source resulting in the reduction of the levels of distillery pollutants and their color by 75.5%. Concomitantly there is a reduction in biological oxygen demand (BOD), chemical oxygen demand (COD), phenol, sulfate, heavy metals. Practical application of this study is the decolorization of the dark distillery effluents. (c) 2006 Elsevier B.V. All rights reserved.