Characterization of posttransplant lymphomas that express T-cell-associated markers: immunophenotypes, molecular genetics, cytogenetics, and heterotransplantation in severe combined immunodeficient mice.
Characterization of posttransplant lymphomas that express T-cell-associated markers: immunophenotypes, molecular genetics, cytogenetics, and heterotransplantation in severe combined immunodeficient mice.
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DOI：
10.1182/blood.v82.1.247.bloodjournal821247
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发表时间：
1993-07
期刊：
影响因子：
20.3
通讯作者：
E. Waller;M. Ziemiańska;C. Bangs;Michael L. Cleary;I. Weissman;O. Kamel
中科院分区：
文献类型：
--
作者：
E. Waller;M. Ziemiańska;C. Bangs;Michael L. Cleary;I. Weissman;O. Kamel
免疫抑制的个体发展为血液恶性肿瘤的高风险。器官移植受者中出现的典型淋巴瘤是含有eb病毒（EBV） DNA序列的b细胞非霍奇金淋巴瘤。我们研究了移植后淋巴瘤的特征，缺乏与EBV转化相关的常见标志物的表达。我们描述了最近在我们机构看到的四个大细胞淋巴瘤。其中2例为CD4+， 1例为CD8+， 1例未进行CD4和CD8表达染色。一例CD4+淋巴瘤为CD30+ EBV-大细胞淋巴瘤，来自一名65岁的肾移植受者；另一例EBV+大细胞淋巴瘤来自一名25岁的心脏移植患者。2例t细胞淋巴瘤为EBV+，并有克隆t细胞受体基因重排。另外两种淋巴瘤表达t细胞标志物CD4和CD43，缺乏b细胞标志物CD19、CD20、CD21、CD22、CD23和表面Ig的表达。两种CD4+淋巴瘤在异源移植到严重联合免疫缺陷（SCID）小鼠体内后都具有致瘤性。SCID小鼠继发性肿瘤的细胞遗传学、免疫表型和基因分型显示其克隆性和与患者原发肿瘤的同一性。从SCID小鼠传代的肿瘤中建立了新的CD4+淋巴瘤细胞系LH521/4和LK418/4。免疫缺陷的环境可能促进这些t细胞或双表型淋巴瘤的生长；其发生的病因学可能包括与EBV和其他尚未确定的机制的转化。
Immunosuppressed individuals are at high risk for the development of hematologic malignancies. The typical lymphomas arising in organ transplant recipients are B-cell non-Hodgkin's lymphomas that contain Epstein-Barr virus (EBV) DNA sequences. We investigated the characteristics of posttransplant lymphomas that lacked expression of the usual markers associated with EBV transformation. We describe four large-cell lymphomas seen recently at our institution. Two of these four cases were CD4+, one was CD8+, and in one staining for CD4 and CD8 expression was not performed. One CD4+ lymphoma was a CD30+, EBV- large-cell lymphoma from a 65-year-old kidney transplant recipient, the second was an EBV+ large-cell lymphoma from a 25-year-old heart transplant patient. Two T-cell lymphomas were EBV+ and had clonal T-cell receptor beta gene rearrangements. The other two lymphomas expressed T-cell markers CD4 and CD43, and lacked expression of B-cell markers CD19, CD20, CD21, CD22, CD23, and surface Ig. Both CD4+ lymphomas were tumorigenic after their heterotransplantation into severe combined immunodeficient (SCID) mice. Cytogenetics, immunophenotyping, and genotyping of the secondary tumors from SCID mice showed their clonality and identity with the patients' primary tumors. Novel CD4+ lymphoma cell lines, LH521/4 and LK418/4, were established from tumors that had been passaged in SCID mice. An immunodeficient environment may facilitate the growth of these T-cell or biphenotypic lymphomas; the etiology of their genesis can include transformation with EBV and other, as yet unidentified mechanisms.