Amino acid analysis.
Amino acid analysis.
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DOI：
10.1002/0471140864.ps1109s58
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发表时间：
2009-11-01
影响因子：
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通讯作者：
Gilani, G Sarwar
中科院分区：
文献类型：
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作者：
Rutherfurd, Shane M;Gilani, G Sarwar
氨基酸分析用于测定含氨基酸、肽和蛋白质样品的氨基酸含量。除了少数例外，蛋白质是通过肽键彼此连接的氨基酸的长线性聚合物。氨基酸分析的第一步涉及水解这些肽键。然后分离、检测和定量释放的氨基酸。该方法最早是由摩尔，斯坦和同事在20世纪50年代使用盐酸水解开发的，尽管许多工人付出了相当大的努力，但基本方法仍然相对不变。该单元提供了氨基酸分析的概述和战略规划，讨论了一系列方法和问题。此外，还详细介绍了几种常用的L-氨基酸分析方法，包括：盐酸水解法、过甲酸氧化法分析蛋氨酸和半胱氨酸、碱水解法分析色氨酸、游离氨基酸分析法和活性赖氨酸分析法。
Amino acid analysis is used to determine the amino acid content of amino acid-, peptide- and protein-containing samples. With minor exceptions, proteins are long linear polymers of amino acids connected to each other via peptide bonds. The first step of amino acid analysis involves hydrolyzing these peptide bonds. The liberated amino acids are then separated, detected, and quantified. The method was first developed by Moore, Stein and coworkers in the 1950s using HCl acid hydrolysis, and, despite considerable effort by many workers, the basic methodology remains relatively unchanged. This unit provides an overview and strategic planning for amino acid analysis, discussing a range of methodologies and issues. In addition, several common methods used for analysis of L-amino acids are described in detail, including: HCl acid hydrolysis, performic acid oxidation for methionine and cysteine analysis, base hydrolysis for tryptophan analysis, analysis of free amino acids, and analysis of reactive lysine.