Effects of glucose on cloning efficiency and mutagenesis of fetal rat cells.
Effects of glucose on cloning efficiency and mutagenesis of fetal rat cells.
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葡萄糖对胎鼠细胞克隆效率和诱变的影响。
DOI：
10.1002/tcm.10027
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Alexandrov,ValeriiA
中科院分区：
文献类型：
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作者：
Donovan,PaulJ;Smith,GeorgeT;Riggs,CharlesW;Alexandrov,ValeriiA
在之前的一项研究中，在妊娠期间和分娩后1.5个月，用10%葡萄糖的饮用水治疗大鼠，妊娠第21天经胎盘给予N -甲基- N -亚硝基脲（MNU, 20 mg/kg）导致的肿瘤发病率显著增加，总体肿瘤发病率增加1.6倍。我们通过体内/体外实验研究了葡萄糖是否会对MNU诱导的胚胎F - 344大鼠体细胞突变产生影响。大鼠胎儿在妊娠第16天经胎盘暴露于MNU，从妊娠第7天到第17天暴露于10%葡萄糖溶液。在妊娠第17天分离细胞，以确定克隆效率并选择6 -硫鸟嘌呤抗性HGPRT突变体。单用MNU处理的胎儿细胞的克隆效率为22.6±2.3% s.e.，而MNU+葡萄糖处理的胎儿细胞的克隆效率为27.5±1.6% s.e.，两者差异有统计学意义（P=0.018）。这表明葡萄糖对细胞增殖和存活有影响。MNU处理显著增加了胎儿细胞的突变频率，从每个活细胞的自发值0.4×10−6增加到（8.8±1.8 s.e.,）×10−6 （P=0.0087）。共暴露于MNU和葡萄糖产生的突变频率为0.62±0.05 s.e.，与单独暴露于MNU相比（0.43±0.11 s.e.，P=0.075）增加了1.5倍。综上所述，这些数据表明，妊娠期间的葡萄糖增加了胎儿细胞的增殖/存活，也可能增加了突变率。畸形发生Carcinog。生物工程学报，2002,22:329-334。出版于2002年Wiley - Liss， Inc。
In a previous study, treatment of rats with 10% glucose in the drinking water, as fetuses during gestation and for 1.5 months after delivery, significantly enhanced tumor incidence that resulted from N‐methyl‐N‐nitrosourea (MNU, 20 mg/kg) given transplacentally on gestation day 21, with a 1.6‐fold increase in overall tumor incidence. We investigated whether glucose would have an effect on MNU‐induced mutation in fetal F‐344 rat somatic cells as measured in an in vivo/in vitro assay. Rat fetuses were exposed transplacentally to MNU on gestation day 16 and to a 10% glucose solution from gestation day 7 to day 17. Cells were isolated on gestation day 17 for determination of cloning efficiency and for selection of 6‐thioguanine (6‐TG)‐resistant HGPRT mutants. Cloning efficiency of the fetal cells exposed to MNU alone was 22.6±2.3% S.E., while that for cells from fetuses exposed to MNU+glucose was 27.5±1.6% S.E., which was a significant difference (P=0.018). This indicates an effect of glucose on cell proliferation and survival. MNU treatment significantly increased the mutation frequency of fetal cells from a spontaneous value of 0.4×10−6per viable cell to (8.8±1.8 S.E.,)×10−6 (P=0.0087). The coexposure to MNU and glucose yielded a mutant frequency per plate of 0.62±0.05 S.E., which was a 1.5‐fold increase compared to MNU alone (0.43±0.11 S.E.,P=0.075. In summary, the data indicate that glucose during pregnancy increases proliferation/survival of fetal cells and possibly also mutation rate.Teratogenesis Carcinog. Mutagen. 22:329–334, 2002. Published 2002 Wiley‐Liss, Inc.