In vitro and in vivo anti-herpes simplex virus activity of monogalactosyl diacylglyceride from Coccomyxa sp. KJ (IPOD FERM BP-22254), a green microalga
In vitro and in vivo anti-herpes simplex virus activity of monogalactosyl diacylglyceride from Coccomyxa sp. KJ (IPOD FERM BP-22254), a green microalga
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DOI：
10.1371/journal.pone.0219305
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发表时间：
2019-07-16
期刊：
影响因子：
3.7
通讯作者：
Hayashi, Toshimitsu
中科院分区：
文献类型：
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作者：
Hayashi, Kyoko;Lee, Jung-Bum;Hayashi, Toshimitsu
从Coccomyxa sp. KJ （IPOD FERM BP-22254）中分离到一种具有抗病毒活性的单半乳糖二酰基甘油酯（MGDG）。α -亚麻酸（C18:3）和7,10,13-十六烯酸（C16:3）分别约占MGDG总脂肪酸的72%和23%。MGDG对引起生殖器疱疹的单纯疱疹病毒2型（HSV-2）具有抗病毒活性。用MGDG治疗后，观察到HSV-2形态的物理变化，包括颗粒大小的减小，以及通过电子显微镜评估的病毒包膜的可能损伤。与形态学结果一致，病毒颗粒失去了与宿主细胞结合的能力。在动物模型中，高浓度MGDG对HSV-2无致病性，表明MGDG对HSV-2颗粒具有不可逆的毒力。在hsv -2诱导的生殖器疱疹动物模型中，经阴道给药MGDG通过抑制生殖器腔内的病毒产量和疱疹性病变的形成发挥了预防作用，导致治疗小鼠的存活率高于对照组小鼠给药溶剂。因此，MGDG提供了一种新的预防HSV感染的选择。
A monogalactosyl diacylglyceride (MGDG) was isolated as an antiviral component from Coccomyxa sp. KJ (IPOD FERM BP-22254) via bioassay-guided fractionation. alpha-Linolenic acid (C18:3) and 7,10,13-hexadecatrienoic acid (C16:3) accounted for approximately 72% and 23%, respectively, of the MGDG total fatty acids of the MGDG. The MGDG showed virucidal activity against herpes simplex virus type 2 (HSV-2), a pathogen that causes genital herpes. Physical changes in HSV-2 shape were observed after treatment with MGDG, including a decrease in particle size, and possible damage to the viral envelope, as assessed using electron microscopy. In accordance with the morphological findings, virus particles lost their ability to bind to host cells. HSV-2 treated with high concentrations of MGDG resulted in no pathogenicity in an animal model, indicating that MGDG exhibits irreversible virucidal activity against HSV-2 particles. In the animal model of HSV-2-induced genital herpes, intravaginally administered MGDG exerted a prophylactic effect by suppressing viral yields in the genital cavity and formation of herpetic lesions, resulting in a higher survival rate in treated mice than control mice administered solvent. Thus, MGDG offers a novel prophylactic option against HSV infections.