A new in vitro model for the study of microbial microleakage around dental restorations:: a preliminary qualitative evaluation
A new in vitro model for the study of microbial microleakage around dental restorations:: a preliminary qualitative evaluation
复制标题
DOI：
10.1046/j.1365-2591.2001.00475.x
复制
发表时间：
2001-10-01
影响因子：
5
通讯作者：
Gulabivala, K
中科院分区：
文献类型：
--
作者：
Matharu, S;Spratt, DA;Gulabivala, K
目的建立一种恒深膜发酵罐（CDFF）模拟牙体/修复体界面微生物微渗漏的体外模型。方法将银汞合金牙体置于机加工的牛牙本质圆柱体中，用清漆密封，在牙体/修复体界面周围留出1 mm的周长。将牙本质圆柱体容纳在a. CDFP和300 μ m厚的微观宇宙牙菌斑生长在它们的暴露表面上。用含粘蛋白的人工唾液维持生物膜长达8周。在无菌条件下从CDFF中取出植入物（在1、2、4和8周时），并在分离和取样对置汞合金和牙本质表面之前，使用经验证的方案进行表面去污。扫描电子显微镜（SEM）用于确定细菌聚集体的位置和结构。细菌活力测定的细菌situ.Results在所有采样时间，扫描电镜显示球菌，杆和细丝的汞合金和牙本质表面;一些起源于级联从表面生物膜和延伸到牙齿/修复体的微空间。活体染色显示，大多数的细菌从牙本质和汞合金surfaces.Conclusion是可行的初步调查表明，CDFF可能是一个有价值的工具，在体外研究的动力学周围的微生物微渗漏牙科漂白。
Aim The aim of this study was to develop an in vitro model to replicate microbial microleakage at a tooth/ restoration interface using a constant depth film fermentor (CDFF).Methodology Amalgam restorations were placed in machined bovine dentine cylinders and sealed externally with varnish, leaving a 1-mm perimeter exposed around the tooth/restoration interface. The dentine cylinders were housed in a. CDFP and 300-mum thick microcosm dental plaques were grown over their exposed surfaces. The biofilms were maintained with a mucin-containing artificial saliva for up to 8 weeks. Cylinders were aseptically removed from the CDFF (at 1, 2, 4, & 8 weeks) and surface-decontaminated with validated protocols prior to splitting and sampling of apposing amalgam and dentine surfaces. Scanning electron microscopy (SEM) was used to ascertain the position and structure of the bacterial aggregates. Bacterial viability was determined by vital staining of the bacteria in situ.Results At all sampling times, SEM showed cocci, rods and filaments on both amalgam and dentine surfaces; some originated as cascades from the surface biofilm and extended into the tooth/restoration microspace. Vital staining showed the majority of bacteria from both dentine and amalgam surfaces to be viable.Conclusion This preliminary investigation showed that the CDFF may be a valuable tool for the in vitro study of the dynamics of microbial microleakage around dental restorations.