A Novel Prebiotic Blend Product Prevents Irritable Bowel Syndrome in Mice by Improving Gut Microbiota and Modulating Immune Response.
A Novel Prebiotic Blend Product Prevents Irritable Bowel Syndrome in Mice by Improving Gut Microbiota and Modulating Immune Response.
复制标题
一种新型益生元混合产品通过改善肠道微生物群和调节免疫反应来预防小鼠肠易激综合症
作者：
Chen Q;Ren Y;Lu J;Bartlett M;Chen L;Zhang Y;Guo X;Liu C
肠易激综合征（IBS）是最常见的功能性胃肠道疾病，但仍缺乏有效的预防治疗方法。本研究的目的是确定一种由低聚果糖（FOS）、低聚半乳糖（GOS）、菊粉和花青素组成的新型益生元混合物（PB）是否能有效预防肠易激综合征的发生。我们在动物和细胞中探索了可能的机制。建立C57BL/6小鼠感染后IBS模型，在感染前8周分别给予PB、PB和益生菌菌株预处理。感染后8周，收集肠道组织进行组织形态学、内脏敏感性、屏障功能、促炎细胞因子表达和蛋白质组学分析。收集粪便样本进行微生物群分析。用PB和鼠伤寒沙门菌14028共孵育Caco-2细胞，观察促炎细胞因子的表达。结果显示，PB在感染Caco-2细胞和PI-IBS模型中均能显著降低促炎细胞因子。PB干预可显著改善感染引起的体重下降、紧密连接蛋白Occludin （OCLN）表达降低和菌群组成变化（p < 0.05）。蛋白质组学分析显示，该功能与过氧化物酶体增殖激活受体(PPAR)γ途径有关。
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder yet it still lacks effective prevention therapies. The aim of this study is to determine whether a novel prebiotic blend (PB) composed of fructo-oligosaccharide (FOS), galactooligosaccharide (GOS), inulin and anthocyanins could be effective in preventing the development of IBS. We explored the possible mechanisms both in animal and in cells. Post-infectious IBS models in C57BL/6 mice were established and were pretreated with the PB, PB and probiotic strains 8 weeks in advance of infection. Eight weeks after infection, intestinal tissues were collected for assessing histomorphology, visceral sensitivity, barrier function, pro-inflammatory cytokines expression and proteomics analysis. Fecal samples were also collected for microbiota analysis. The pro-inflammatory cytokines expression in Caco-2 cells were evaluated after co-incubation with PB and Salmonella typhimurium 14028. The results showed that PB significantly decreased the pro-inflammatory cytokines both in infected Caco-2 cells and PI-IBS models. The loss of body weight, decreased expression of tight junction protein Occludin (OCLN), and changes of the microbiota composition induced by infections could be greatly improved by PB intervention (p < 0.05). The proteomics analysis revealed that this function was associated with Peroxisome proliferator-activated receptor (PPAR)γ pathway.
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影响因子：
3.9
作者：
Canavan C;West J;Card T
通讯作者：
Card T
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
3.7
作者：
Gomez A;Luckey D;Yeoman CJ;Marietta EV;Berg Miller ME;Murray JA;White BA;Taneja V
通讯作者：
Taneja V
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
11.8
作者：
Doron, Shira;Snydman, David R.
通讯作者：
Snydman, David R.