Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa.
Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa.
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由粗糙脉孢菌无转化菌株表达的节律的昼夜节律性质。
DOI：
10.1104/pp.41.8.1343
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发表时间：
1966
期刊：
影响因子：
7.4
通讯作者：
D. Woodward
中科院分区：
文献类型：
--
作者：
M. Sargent;W. Briggs;D. Woodward
本文描述了一种粗糙脉孢菌的新菌株，它在生长管中沿琼脂表面沿着生长时表现出分生孢子的节律。这种节律被证明是昼夜节律，因为它符合以下标准：A）在黑暗中恒定环境条件下的周期约为24小时（25度时为22.7小时）; B）该周期相对与温度无关（对于18至35度的温度范围，Q（10）在0.95至1.21之间）; C）在恒定温度下连续黑暗中，节律持续至少14天而不衰减;和D）节律的相位可以通过单一短暂暴露于光而改变。该菌株对光的敏感性已经通过使用合适的光-暗方案将节律夹带到24.0小时的时间段以及通过光抑制节律的出现而进一步证明;即，当菌株受到连续光照时，发生连续分生孢子。将新菌株与另外两种同样表达节律、斑块和时钟的脉孢菌菌株进行比较。
A new strain of Neurospora crassa which exhibits a rhythm of conidiation when growing along an agar surface in a growth tube is described. The rhythm has been shown to be circadian for it meets the following criteria: A) the period under constant environmental conditions in the dark is about 24 hours (22.7 hours at 25 degrees ); B) the period is relatively temperature-independent (Q(10) is between 0.95 and 1.21 for temperature range of 18 to 35 degrees ); C) the rhythm persists in continuous darkness at constant temperature for a minimum of 14 days without damping out; and D) the phase of the rhythm can be shifted by a single brief exposure to light. The sensitivity of this strain to light has been demonstrated further by the entrainment of the rhythm to a period of 24.0 hours using a suitable light-dark regime, and by the inhibition by light of the appearance of a rhythm; i.e., continuous conidiation occurs when the strain is subjected to continuous light. The new strain is compared to 2 other strains of Neurospora which also express a rhythm, patch and clock.