Comparative effects of NaCl and NaHCO3 stress on photosynthetic parameters, nutrient metabolism, and the antioxidant system in tomato leaves
Comparative effects of NaCl and NaHCO3 stress on photosynthetic parameters, nutrient metabolism, and the antioxidant system in tomato leaves
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DOI：
10.1016/j.scienta.2013.03.032
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发表时间：
2013-06-20
影响因子：
4.3
通讯作者：
Wang, Xiufeng
中科院分区：
文献类型：
--
作者：
Gong, Biao;Wen, Dan;Wang, Xiufeng
比较了NaCl和NaHCO_3胁迫对番茄的伤害效应。随着胁迫强度的增加，NaCl和NaHCO 3处理下番茄地上部和根系的生物量积累均显着下降。与NaCl胁迫相比，NaHCO 3胁迫对番茄生长的抑制作用更大，这与NaHCO 3胁迫对光合作用的抑制作用更强有关。叶绿素（Chl）含量、可变荧光与最大荧光的比值（F-v/F-m）和光合系统II的实际量子效率（phi PSII）均随NaCl和NaHCO 3浓度的增加而降低，且NaHCO 3处理的降低幅度更大。相反，随着NaCl和NaHCO 3浓度的增加，非光化学猝灭（NPQ）增加，尤其是NaHCO 3处理。番茄叶片Na含量与NaCl和NaHCO 3胁迫强度呈正相关，K、P、N含量及氮代谢相关酶硝酸还原酶（NR）、谷氨酰胺合成酶（GS）、谷氨酸脱氢酶（GDH）和谷氨酸合成酶（GOGAT）含量与胁迫强度呈显著负相关，且NaHCO 3处理的变化幅度远大于NaCl处理。NaCl和NaHCO 3胁迫均能诱导番茄叶片活性氧（ROS）的过量积累和明显的膜脂过氧化作用，但NaHCO 3处理的活性氧积累和膜脂过氧化作用明显高于NaCl处理。与过氧化氢酶（CAT）和愈创木酚过氧化物酶（GPX）相比，超氧化物歧化酶（SOD）以及抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（达尔）和谷胱甘肽还原酶（GR）等抗坏血酸-谷胱甘肽循环组分在盐碱胁迫下表现出较高的活性，可能在清除活性氧方面发挥重要作用。(C)2013爱思唯尔有限公司版权所有。
Detrimental effects of NaCl and NaHCO3 stress on tomato plants were compared. With increasing stress intensity, accumulation of biomass in tomato shoots and roots significantly decreased in both NaCl and NaHCO3 treatments. Compared to NaCl stress, NaHCO3 stress showed greater reduction of tomato plant growth, which was in accordance with stronger inhibition of photosynthesis by NaHCO3 stress. Chlorophyll (Chl) contents, the ratio of the variable fluorescence to the maximum fluorescence(F-v/F-m) and the actual quantum efficiency of photosynthetic system II (phi PSII) declined with increasing concentration of NaCl and NaHCO3, and their decreasing degree was more significant in NaHCO3 treatment. On the contrary, higher non-photochemical quenching (NPQ) was observed with increasing concentration of NaCl and NaHCO3, especially in NaHCO3 treatment. Na content in tomato leaves was positively correlated with NaCl and NaHCO3 stress intensity, while contents of K, P, and N as well as nitrogen metabolism-related enzymes including nitrate reductase (NR), glutamine synthetase (GS), glutamate dehydrogenase (GDH) and glutamate synthase (GOGAT) showed significant negative correlation with stress intensity, and their changes were much higher in NaHCO3 treatment than in NaCl treatment. Both NaCl and NaHCO3 stress induced excess reactive oxygen species (ROS) accumulation and obvious lipid peroxidation in tomato leaves, though was much higher in the NaHCO3 treatment. Compared to catalase (CAT) and guaiacol peroxidase (GPX), superoxide dismutase (SOD) and the ascorbate-glutathione cycle components including ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) showed higher levels, which could play an important role in scavenging ROS in tomato leaves under salt and alkali stress. (C) 2013 Elsevier B.V. All rights reserved.