Investigations of a W-Rh permanent modifier for the determination of Pb in blood by electrothermal atomic absorption spectrometry
Investigations of a W-Rh permanent modifier for the determination of Pb in blood by electrothermal atomic absorption spectrometry
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DOI：
10.1016/s0584-8547(02)00085-x
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发表时间：
2002-08
期刊：
影响因子：
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通讯作者：
Ying Zhou;Renato A. Zanão;Fernando Barbosa;P. Parsons;F. Krug
中科院分区：
文献类型：
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作者：
Ying Zhou;Renato A. Zanão;Fernando Barbosa;P. Parsons;F. Krug
在横向加热石墨原子化器的集成平台上，用钨-铑涂层(2 5 0μg W+2 0 0μg Rh)作为永久性化学改进剂，用电热原子吸收光谱法直接测定全血中铅。血液样品用0.2%v/v HNO3和0.5%v/v TritonX-100稀释液稀释1+9，将稀释后的L 12μ放在平台上。使用W-Rh改进剂后，与传统的NH_4H_2PO_4改进剂相比，管寿命提高了2~3倍。对于标准的THGA管，使用W-Rh永久改进剂可以用水标准溶液进行简单的校准，用于血铅测量。然而，当使用端盖THGA管时，矩阵匹配校准仍然是必要的。以积分吸光度为基础的方法检出限(3σ)为1.5molgdl−1(0.092μmol1−1)，端盖试管为1.0molgdl−1(0.048μmol1−1)。为了验证的目的，对各种血基认证标准物质和铅认证的能力测试样本进行了分析。所有结果都在被认为可以接受的范围内。针对已建立的使用磷酸盐改进剂的血铅ETAAS方法，对用于血铅测量的人体血样进行分析提供了额外的验证数据。
A tungsten-rhodium coating (250 μg W+200 μg Rh) on the integrated platform of a transversely heated graphite atomizer (THGA) was used as a permanent chemical modifier for the direct determination of lead in whole blood by electrothermal atomic absorption spectrometry (ETAAS). Blood samples were diluted 1+9 with a diluent consisting of 0.2% v/v HNO3and 0.5% v/v TritonX-100, and 12 μl of the diluted sample was deposited on the platform. With use of the W-Rh modifier, tube lifetime was improved by a factor of 2–3 when compared with a conventional NH4H2PO4modifier. With a standard THGA tube, use of the W-Rh permanent modifier allows simple calibration with aqueous standard solutions for blood Pb measurement. However, matrix-matched calibration is still necessary when an end-capped THGA tube is used. The method detection limit (3σ) based on integrated absorbance measurements is 1.5 μg dl−1(0.092 μmol 1−1) for the standard THGA and 1.0 μg dl−1(0.048 μmol l−1) for the end-capped tube. Various blood-based certified reference materials and proficiency testing samples, certified for Pb, were analyzed for validation purposes. All results were well within the range considered acceptable. Analysis of human blood samples for blood Pb measurement provided additional validation data against an established blood Pb ETAAS method that uses phosphate modifier.