The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos
The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos
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体外受精与单亲植入前猪胚胎染色质3D结构的异步建立
DOI：
10.1186/s13059-020-02095-z
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发表时间：
2020
期刊：
影响因子：
12.3
通讯作者：
Wang Yanfang
中科院分区：
文献类型：
--
作者：
Li Feifei;Wang Danyang;Song Ruigao;Cao Chunwei;Zhang Zhihua;Wang Yu;Li Xiaoli;Huang Jiaojiao;Liu Qiang;Hou Naipeng;Xu Bingxiang;Li Xiao;Gao Xiaomeng;Jia Yan;Zhao Jianguo;Wang Yanfang
背景猪是农业和生物医学研究的重要动物，辅助生殖技术的应用需要改进。确定体外受精（IVF）、孤雌生殖和雄核发育所产生的植入前胚胎早期表观遗传重编程的潜在机制不仅有助于猪的辅助生殖技术，也将有助于人类早期发育。然而，重编程的三维结构的染色质在这个过程中，在pigs.ResultsWe产生的三维染色质的猪体细胞，体外受精，孤雌生殖，和雄核发育植入前胚胎。我们发现猪植入前胚胎的染色体富含超结构域，这在小鼠中更为罕见。然而，p（s）曲线，隔间，拓扑相关结构域（TADs）在体细胞中很大程度上是保守的，并逐渐建立在植入前胚胎发生在两种哺乳动物。在单亲猪胚胎中，染色质结构的建立在IVF胚胎的所有水平上都是高度分离的，并且在合子基因组激活（ZGA）期间观察到非常强烈的去部分化。最后，来自卵母细胞的染色体总是比来自精子的染色体更快地建立TADs，无论是在ZGA之前还是在ZGA期间。暗示猪的单亲胚胎中ZGA严重失调。
BackgroundPigs are important animals for agricultural and biomedical research, and improvement is needed for use of the assisted reproductive technologies. Determining underlying mechanisms of epigenetic reprogramming in the early stage of preimplantation embryos derived from in vitro fertilization (IVF), parthenogenesis, and androgenesis will not only contribute to assisted reproductive technologies of pigs but also will shed light into early human development. However, the reprogramming of three-dimensional architecture of chromatin in this process in pigs is poorly understood.ResultsWe generate three-dimensional chromatin profiles for pig somatic cells, IVF, parthenogenesis, and androgenesis preimplantation embryos. We find that the chromosomes in the pig preimplantation embryos are enriched for superdomains, which are more rare in mice. However, p(s) curves, compartments, and topologically associated domains (TADs) are largely conserved in somatic cells and are gradually established during preimplantation embryogenesis in both mammals. In the uniparental pig embryos, the establishment of chromatin architecture is highly asynchronized at all levels from IVF embryos, and a remarkably strong decompartmentalization is observed during zygotic genome activation (ZGA). Finally, chromosomes originating from oocytes always establish TADs faster than chromosomes originating from sperm, both before and during ZGA.ConclusionsOur data highlight a potential unique 3D chromatin pattern of enriched superdomains in pig preimplantation embryos, an unusual decompartmentalization process during ZGA in the uniparental embryos, and an asynchronized TAD reprogramming between maternal and paternal genomes, implying a severe dysregulation of ZGA in the uniparental embryos in pigs.