Nocturnal hypertension in mice consuming a high fructose diet
Nocturnal hypertension in mice consuming a high fructose diet
复制标题
DOI：
10.1016/j.autneu.2006.05.006
复制
发表时间：
2006-12-30
影响因子：
2.7
通讯作者：
Morris, Mariana
中科院分区：
文献类型：
--
作者：
Farah, Vera;Elased, Khalid M.;Morris, Mariana
目的：探讨果糖摄入对小鼠血压、心率和自主神经功能的光/暗模式的影响。背景：胰岛素抵抗型糖尿病与高血压和自主神经功能障碍有关。有证据表明，糖尿病发病率的增加可能与饮食变化有关，包括摄入高水平果糖。 设计/方法：使用无线电遥测动脉导管对 C57/BL 小鼠进行对照或高果糖饮食 (60%) 喂养。测量的心血管参数是平均动脉压（MAP）、心率（HR）和变异性（时域和频域）的明/暗模式。我们还测量了血浆胰岛素、葡萄糖、血脂和血管紧张素 11 (Ang 11) 以及葡萄糖耐量。原位杂交用于测量脑干酪氨酸羟化酶（TH）和Ang AT1a mRNA的表达。结果：果糖饮食（8周）产生MAP、方差和低频域的增加（14 3 vs. 3314 min Hg-2，方差和2 10 2 vs. 26 4 mm Hg，LF，对照与果糖，P < 0.01）。这些变化仅发生在夜间，即老鼠活动的时期。果糖组的葡萄糖耐量减弱。果糖还增加血浆胆固醇（80 1 vs. 126 2 mg/dl，对照与果糖，P < 0.05）和血浆血管紧张素II（18 +/- 5 vs.65 +/- 12 pg/ml，对照与果糖，P < 0.05）。在果糖喂养的小鼠中，普拉索嗪对 α(1)-肾上腺素能阻断的抑制反应增强。采用定量原位杂交技术，我们发现脑干蓝斑中Ang AT1a受体和TH mRNA表达显着增加。结论：高果糖饮食导致小鼠出现夜间高血压和自主神经失衡，可能与交感神经系统和血管紧张素系统的激活有关。 (c) 2006 Elsevier B.V. 保留所有权利。
Objective: To investigate the effect of fructose consumption on the light/dark pattern of blood pressure, heart rate and autonomic neural function in mice.Background: Insulin resistant diabetes is associated with hypertension and autonomic dysfunction. There is evidence that the increasing incidence of diabetes may be related to dietary changes, including consumption of high levels of fructose.Design/methods: C57/BL mice, instrumented with radiotelemetric arterial catheters, were fed a control or high fructose diet (60%). Cardiovascular parameters measured were light/dark pattern of mean arterial pressure (MAP), heart rate (HR) and variability (time and frequency domain). We also measured plasma insulin, glucose, lipids and angiotensin 11 (Ang 11) as well as glucose tolerance. In situ hybridization was used to measure brainstem expression of tyrosine hydroxylase (TH) and Ang AT1a mRNA.Results: Fructose diet (8 weeks) produced an increase in MAP, variance and low frequency domain (14 3 vs. 3314 min Hg-2, variance and 2 10 2 vs. 26 4 mm Hg, LF, control vs. fructose, P < 0.01). The changes occurred only,at night, a period of activity for mice. Glucose tolerance was attenuated in the fructose group. Fructose also increased plasma cholesterol (80 1 vs. 126 2 mg/dl, control vs. fructose, P < 0.05) and plasma Ang II (18 +/- 5 vs.65 +/- 12 pg/ml, control vs. fructose, P < 0.05). Depressor responses to alpha(1)-adrenergic blockade with prasozin were augmented in fructose-fed mice. Using quantitative in situ hybridization, we found that Ang AT1a receptor and TH mRNA expression were significantly increased in the brainstem locus coeruleus.Conclusion: A high fructose diet in mice produced nocturnal hypertension and autonomic imbalance which may be related to activation of sympathetic and angiotensin systems. (c) 2006 Elsevier B.V. All rights reserved.