Zinc-secreting Paneth cells studied by ZP fluorescence
Zinc-secreting Paneth cells studied by ZP fluorescence
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DOI：
10.1369/jhc.5a6724.2005
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发表时间：
2006-03-01
影响因子：
3.2
通讯作者：
Frederickson, CJ
中科院分区：
文献类型：
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作者：
Giblin, LJ;Chang, CJ;Frederickson, CJ
我们使用了一系列新的锌特异性响应荧光染料（ZP）来研究潘氏细胞中锌的螯合和分泌，潘氏细胞位于大鼠小肠内利伯库恩隐窝的基部。在低温恒温器切片和分离的隐窝中都可以看到潘氏细胞分泌颗粒的生动 ZP 荧光锌染色，为这些细胞内存在不稳定（快速交换）的锌池提供了有力的证据。我们进一步证明这种离子锌库是在生理条件下分泌的。通过腹膜内注射促分泌剂毛果芸香碱对小肠进行体内刺激，导致随后分离的隐窝内腔内出现离散的锌染色，同时位于同一隐窝内的潘氏细胞颗粒的锌染色减少。相反，在体外用卡巴胆碱或LPS（脂多糖）刺激的分离隐窝的管腔中没有观察到锌的分泌。然而，潘氏细胞形态发生明显变化，表明尝试分泌，是对直接应用胆碱能药物而非 LIPS 的反应。这些发现表明，锌与其他潘氏细胞抗微生物剂共同释放，并且完整的肠道对于分泌到隐窝腔是必需的。
We have used a new family of zinc-specific-responsive fluorescent dyes (ZPs) to study the sequestration and secretion of zinc from Paneth cells, which are located in the bases of the crypts of Lieberkuhn within the rat small intestine. Vivid ZP fluorescence zinc staining of Paneth cell secretory granules is seen in both cryostat sections and isolated crypts, providing firm evidence for a pool of labile (rapidly exchangeable) zinc within these cells. We further demonstrate that this ionic zinc pool is secreted under physiological conditions. In vivo stimulation of the small intestine by IP injection of the secretagogue pilocarpine results in discrete zinc staining within the lumens of subsequently isolated crypts, concomitant with a decrease in the zinc staining of Paneth cell granules located within the same crypts. In contrast, the secretion of zinc into the lumens of isolated crypts stimulated in vitro I with either carbachol or LPS (lipopolysaccharide) is not observed. However, a distinct change in Paneth cell morphology, suggesting attempted secretion, is seen in response to the direct application of cholinergics but not LIPS. These findings suggest that zinc is coreleased with other Paneth cell anti-microbials, and that the intact intestine is necessary for secretion into the crypt lumen.