The quorum sensing luxS gene is induced in Lactobacillus acidophilus NCFM in response to Listeria monocytogenes
The quorum sensing luxS gene is induced in Lactobacillus acidophilus NCFM in response to Listeria monocytogenes
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DOI：
10.1016/j.ijfoodmicro.2011.06.011
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发表时间：
2011-10-03
影响因子：
5.4
通讯作者：
Jespersen, Lene
中科院分区：
文献类型：
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作者：
Moslehi-Jenabian, Saloomeh;Vogensen, Finn Kvist;Jespersen, Lene
参与群体感应的LuxS基因已被证明控制益生菌的不同行为。在这项研究中，我们研究了嗜酸乳杆菌NCFM中的LuxS是否在单核细胞增生性李斯特菌EGD-e反应中上调。将这两株细菌分别在单独和联合培养条件下进行培养，观察两株细菌在L嗜酸细胞中的生长情况和LUX S的转录水平。与L嗜酸菌相反，共培养显著影响L单核细胞增殖菌的生长。转录分析表明，在L嗜酸细胞中，LuxS的表达在指数生长期呈上升趋势，指数生长期晚期表达最高，稳定期下降。与L单核细胞共培养后，与活的L单核细胞孵育和添加L单核细胞无细胞培养上清后，嗜酸L指数期中期细胞的LuxS转录水平显著增加，而与L单核细胞热灭活细胞孵育对其转录水平无明显影响。这可能表明LUXS的上调是由于对L单核细胞产生的一种分泌性化合物的反应。(C)2011爱思唯尔B.V.保留所有权利。
The luxS gene involved in quorum sensing has been shown to control different behaviour of probiotic lactobacilli. In this study we investigated if luxS in Lactobacillus acidophilus NCFM was up-regulated in response to Listeria monocytogenes EGD-e. The two bacterial strains were grown in mono- and co-culture and the growth of both bacteria and the transcriptional level of luxS in L acidophilus cells were monitored. Contrary to L acidophilus, the growth of L monocytogenes was significantly affected by co-cultivation. Transcriptional analysis showed that the expression of luxS increased during exponential growth in L acidophilus cells with the highest level in the late-exponential growth phase, decreasing in the stationary phase. Following co-cultivation with L monocytogenes, the transcriptional level of luxS increased significantly in mid-exponential growing cells of L acidophilus after incubation with viable L monocytogenes cells and by addition of cell-free culture supernatant of L monocytogenes, whereas incubation with heat killed cells of L monocytogenes had no effect on the transcriptional level. This could indicate that the up-regulation of luxS is due to a response to a secreted compound produced by L monocytogenes cells. (C) 2011 Elsevier B.V. All rights reserved.