Comparative Phylogenetic Relationships and Genetic Structure of the Caterpillar Fungus Ophiocordyceps sinensis and Its Host Insects Inferred from Multiple Gene Sequences
Comparative Phylogenetic Relationships and Genetic Structure of the Caterpillar Fungus Ophiocordyceps sinensis and Its Host Insects Inferred from Multiple Gene Sequences
复制标题
DOI：
10.1007/s12275-014-3391-y
复制
发表时间：
2014-02-01
影响因子：
3
通讯作者：
Cheng, Zhou
中科院分区：
文献类型：
--
作者：
Quan, Qing-Mei;Wang, Qing-Xia;Cheng, Zhou
冬虫夏草（Ophiocordyceps sinensis，子囊菌纲：蛇虫夏草科）是一种天然寄生于Hepialidae毛虫的真菌，是中国最重要的药用毛虫真菌之一。然而，人们对中华夜蛾与寄主昆虫的系统发育和进化关系知之甚少。本研究对中国5个地区33个种群样本进行核ITS和β -微管蛋白序列分析，并对其宿主线粒体COI、COII和Cytb序列进行分析。在系统发育上，中华按蚊及其寄主可划分为3个地理上相关的支系，且系统发育一致。分子变异分析和遗传分化系数计算结果表明，中华白颡鱼（F-ST=0.878, N-ST=0.842）与寄主（F-ST=0.861, N-ST=0.816）之间存在显著的遗传差异。在这3个支系中，中华夜蛾（Nm=0.04）和寄主昆虫（Nm=0.04）的估计基因流量都很低。Mantel检验显示两者有显著相关性(P
Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae) is a native fungal parasite of Hepialidae caterpillars and one of the most economically important medicinal caterpillar fungi in China. However, little is known about the phylogenetic and evolutionary relationships between O. sinensis and its host insects. In this study, nuclear ITS and beta-tubulin sequences from O. sinensis and mitochondrial COI, COII, and Cytb sequences from its hosts were analyzed across 33 populations sampled from five regions in China. Phylogenetically, both O. sinensis and its hosts were divided into three geographically correlated clades, and their phylogenies were congruent. Analysis of molecular variance and calculated coefficients of genetic differentiation revealed significant genetic divergence among the clades within both O. sinensis (F-ST=0.878, N-ST=0.842) and its hosts (F-ST=0.861, N-ST=0.816). Estimated gene flow was very low for O. sinensis (Nm=0.04) and the host insects (Nm=0.04) among these three clades. Mantel tests demonstrated a significant correlation (P