A modification of Mallory's connective tissue stain with a discussion of the principles involved
A modification of Mallory's connective tissue stain with a discussion of the principles involved
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DOI：
10.1002/ar.1090690105
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发表时间：
1937-08-01
影响因子：
--
通讯作者：
Crossmon, G
中科院分区：
文献类型：
--
作者：
Crossmon, G
自1900年马洛里苯胺蓝结缔组织染色法发表以来，人们提出了许多改进方法。然而，这些方法通常产生通常不令人满意的结果，或者在诸如红细胞和骨的元素的着色方面不一致。在某些情况下，修改表明对该方法的基本原则缺乏理解;在另一些情况下，困难仅仅是由诸如过分严格地遵守切片在各种试剂中浸泡的时间规定、未能避免溶液耗尽等实际错误引起的。不理解这些点，即使是最初的马洛里方法也不能产生恒定的结果。本文所述的方法和所附注释中所解释的方法考虑了基本原理，并产生了均匀良好染色的制备物。马洛里最初的方法是将适当固定的切片用酸性品红染色，这种品红通常会使组织呈现出或多或少均匀的红色。然后用磷钼酸从胶原纤维上除去这种染色剂，磷钼酸同时作为随后用苯胺蓝染色相同纤维的媒染剂。橙子G与苯胺蓝混合，用作髓鞘和红血球的染色剂。当成功时，结缔组织网，胶原纤维，粘液，淀粉样蛋白
Since the publication in 1900 of Mallory's anilin blue connective tissue stain, a number of modifications have been proposed. These methods, however, often yield results which are generally unsatisfactory, or else discrepant with regard to the coloration of such elements as red blood corpuscles and bone. In some cases the modifications reveal lack of comprehension of the fundamental principles of the method; in others, the difficulty is caused merely by such practical errors as over-strict adherence to a time stipulation for immersion of sections in the various reagents, failure to avoid exhaustion of the solutions, etc. Without understanding of these points, not even the original Mallory method will yield constant results.The procedure described in this paper and explained in the accompanying notes takes account of the basic principles and results in uniformly well-stained preparations. Mallory's original method consists in staining appropriately fixed sections in acid fuchsin, which confers a more or less uniform red color upon the tissue generally. This stain is then removed from the collagenous fibers by phosphomolybdic acid, which at the same time acts as a mordant for subsequent staining of the same fibers with anilin blue. Orange G mixed with the anilin blue, serves as a stain for myelin sheaths and red blood corpuscles. When successful, connective tissue reticulum, collagenous fibers, mucus, amyloid