A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling
A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling
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DOI：
10.1126/science.1093586
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发表时间：
2004-03-05
期刊：
影响因子：
56.9
通讯作者：
Takayama, S
中科院分区：
文献类型：
--
作者：
Murase, K;Shiba, H;Takayama, S
甘蓝型油菜的自交不亲和反应是由花粉携带的配基S位点蛋白11/Scr与柱头S受体激酶SRK之间的单倍型特异性相互作用启动的。这种结合诱导了SRK的自磷酸化，然后被认为触发了导致自交排斥的信号级联反应。修饰(M)基因的隐性突变消除了柱头的SI反应。M基因的定位克隆表明，它编码一种膜锚定的细胞质丝氨酸/苏氨酸蛋白激酶，称为M基因座蛋白激酶(MLPK)。MLPK的瞬时表达恢复了mm乳头细胞拒绝自交花粉的能力，这表明MLPK是油菜SI信号的一个积极的中介。
Self-incompatibility (SI) response in Brassica is initiated by haplotype-specific interactions between the pollen-borne ligand S locus protein 11/SCR and its stigmatic S receptor kinase, SRK. This binding induces autophosphorylation of SRK, which is then thought to trigger a signaling cascade that leads to self-pollen rejection. A recessive mutation of the modifier (m) gene eliminates the SI response in stigma. Positional cloning of M has revealed that it encodes a membrane-anchored cytoplasmic serine/threonine protein kinase, designated M locus protein kinase (MLPK). Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling.