Population structure of wild bananas, Musa balbisiana, in China determined by SSR fingerprinting and cpDNA PCR-RFLP
Population structure of wild bananas, Musa balbisiana, in China determined by SSR fingerprinting and cpDNA PCR-RFLP
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DOI：
10.1111/j.1365-294x.2005.02467.x
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发表时间：
2005-04-01
影响因子：
4.9
通讯作者：
Chiang, TY
中科院分区：
文献类型：
--
作者：
Ge, XJ;Liu, MH;Chiang, TY
种群历史和扩散机制都影响着不同地理区域植物种群间遗传多样性的分配。本研究采用简单重复序列指纹图谱和cpDNAPCRRFLP技术，对中国栽培香蕉和车前草的祖先之一--野生香蕉的系统地理和种群结构进行了研究。21个单倍型的叶绿体DNA(CpDNA)系谱分析表明，它们有两个主要分支，分别对应于北江和西江隔开的两个地理区域，这表明它们之间存在着更替的历史。利用cpDNA标记检测到群体间存在显著的遗传分化，这一结果与啮齿动物觅食介导的种子在野生香蕉中的有限传播一致。核SSR数据还揭示了香蕉种群的显著地理结构。然而，在中国西部，没有发现系统地理格局，这可能是由于花粉频繁地通过果蝠流动所致。相比之下，北江以东的种群和海南岛的种群在基因上是截然不同的，因为那里没有远距离飞翔的传粉者。定居-灭绝过程可能影响了Musa种群的进化，Musa种群具有集合种群结构，并通过迁徙的个体联系在一起。根据核SSR数据估计的通过花粉的有效基因流动，是根据cpDNA数据估计的通过种子的基因流动的3.65倍。叶绿体和核DNA对野生香蕉种群的系统地理模式提供了不同的见解，结合在一起，可以为保护实践提供信息。
Both demographic history and dispersal mechanisms influence the apportionment of genetic diversity among plant populations across geographical regions. In this study, phylogeography and population structure of wild banana, Musa balbisiana, one of the progenitors of cultivated bananas and plantains in China were investigated by an analysis of genetic diversity of simple sequence repeat (SSR) fingerprint markers and cpDNA PCR-RFLP. A chloroplast DNA (cpDNA) genealogy of 21 haplotypes identified two major clades, which correspond to two geographical regions separated by the Beijiang and Xijiang rivers, suggesting a history of vicariance. Significant genetic differentiation was detected among populations with cpDNA markers, a result consistent with limited seed dispersal in wild banana mediated by foraging of rodents. Nuclear SSR data also revealed significant geographical structuring in banana populations. In western China, however, there was no detected phylogeograpahical pattern, possibly due to frequent pollen flow via fruit bats. In contrast, populations east of the Beijiang River and the population of Hainan Island, where long-range soaring pollinators are absent, are genetically distinct. Colonization-extinction processes may have influenced the evolution of Musa populations, which have a metapopulation structure and are connected by migrating individuals. Effective gene flow via pollen, estimated from the nuclear SSR data, is 3.65 times greater than gene flow via seed, estimated from cpDNA data. Chloroplast and nuclear DNAs provide different insights into phylogeographical patterns of wild banana populations and, taken together, can inform conservation practices.