The effect of environmental factors on the response of human corneal epithelial cells to nanoscale substrate topography
The effect of environmental factors on the response of human corneal epithelial cells to nanoscale substrate topography
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DOI：
10.1016/j.biomaterials.2006.01.044
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Murphy, CJ
中科院分区：
文献类型：
--
作者：
Teixeira, AI;McKie, GA;Murphy, CJ
我们先前已经表明，人角膜上皮细胞感知纳米级基底地形刺激并对其作出反应[特谢拉Al，Abrams GA，Bertics PJ，Murphy CJ，Nealey PF. J Cell Sci 2003-116（10）：1881-92; Karuri NW，Liliensiek S，特谢拉Al，Abrams G，坎贝尔S. Nealey PF等人，生物学长度尺度地形图增强人角膜上皮细胞的细胞-基质粘附。J Cell Sci 2004.117（15）：3153-641.在这里，我们证明了细胞对纳米级基底形貌的反应是由这些刺激呈现给细胞的背景所调制的。在Epilife培养基中，细胞优先在垂直于纳米级凹槽和脊的方向上排列。这与先前的研究相反，在先前的研究中，在DMEM/F12培养基中培养的细胞在平行于纳米级形貌特征的方向上排列[特谢拉Al，Abrams GA，Bertics PJ，Murphy CJ，Nealey PF. Epithelia]，在良好限定的微米和纳米结构基底上接触引导。J Cell Sci 2003，- 116（10）：1881-92]。此外，Epilife培养基中的细胞排列取决于图案间距。当间距从400 nm增加到4000 nm时，细胞从垂直排列切换到平行排列。有一个过渡区（800和1600纳米间距之间），平行和垂直对齐相比，所有其他细胞的取向是有利的。细胞在该过渡区形成平行于基底形貌的局灶性粘附。在纳米和微米尺度的图案，400和4000 nm的间距，焦点粘连几乎完全倾斜的地形图案。(c)2006爱思唯尔有限公司保留所有权利。
We have previously shown that human corneal epithelial cells sense and react to nanoscale substrate topographic stimuli [Teixeira Al, Abrams GA, Bertics PJ, Murphy CJ, Nealey PF. Epithelial contact guidance on well-defined micro- and nanostructured substrates. J Cell Sci 2003-116(10):1881-92; Karuri NW, Liliensiek S, Teixeira Al, Abrams G, Campbell S. Nealey PF, et al. Biological length scale topography enhances cell-substratum adhesion of human corneal epithelial cells. J Cell Sci 2004.117(15):3153-641. Here we demonstrate that cellular responses to nanoscale Substrate topographies are modulated by the context in which these stimuli are presented to cells. In Epilife medium, cells aligned preferentially in the direction perpendicular to nanoscale grooves and ridges. This is in contrast to a previous Study where cells cultured in DMEM/F12 medium aligned in the direction parallel to nanoscale topographic features [Teixeira Al, Abrams GA, Bertics PJ, Murphy CJ, Nealey PF. Epithelia] contact guidance on well-defined micro- and nanostructured substrates. J Cell Sci 2003,- 116(10):1881-92]. Additionally, cell alignment in Epilife medium was dependent on pattern pitch. Cells switched from perpendicular to parallel alignment when the pitch was increased from 400 to 4000 nm. There was a transition region (between 800 and 1600 nm pitch) where both parallel and perpendicular alignments were favored compared to all other cellular orientations. Cells formed focal adhesions parallel to the substrate topographies in this transition region. On the nano- and microscale patterns, 400 and 4000nm pitch, focal adhesions were almost exclusively oriented obliquely to the topographic patterns. (c) 2006 Elsevier Ltd. All rights reserved.