Transcriptome and proteomic analyses reveal multiple differences associated with chloroplast development in the spaceflight-induced wheat albino mutant mta.
Transcriptome and proteomic analyses reveal multiple differences associated with chloroplast development in the spaceflight-induced wheat albino mutant mta.
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DOI：
10.1371/journal.pone.0177992
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Shi K;Gu J;Guo H;Zhao L;Xie Y;Xiong H;Li J;Zhao S;Song X;Liu L
叶绿体发育是植物生存和生长的一个组成部分，与叶绿素生物合成平行发生。然而，对六倍体小麦叶绿体发育的机制知之甚少。在这里，我们获得了一个航天诱变小麦白化病突变体。叶绿体超微结构的观察表明，与野生型相比，野生型的叶绿体形态和分布异常。光合色素含量也显著降低。分别进行了拟南芥和野生型的转录组和叶绿体蛋白质组分析，以鉴定差异表达的基因（DEG）和蛋白质（DEP）。结果表明，在拟南芥中共检测到4,588个DEG，其中上调1,980个，下调2,608个;叶绿体DEPs共检测到48个，其中上调15个，下调33个。DEG的分类显示，大多数参与叶绿体发育、叶绿素生物合成或光合作用。此外，还鉴定了PIF 3、GLK和MYB等可能参与这些途径的转录因子。DEGs与DEPs之间的相关性分析表明，DEGs与DEPs之间存在着大量的蛋白质转换功能，主要参与光合作用和叶绿体相关功能。真实的时间qPCR分析证实，编码光合蛋白的基因的表达水平显着降低，在拟南芥。总之，我们的研究结果表明，白化病叶色形成的分子机制是一个彻底调节和复杂的过程。转录组和蛋白质组的联合分析为进一步研究小麦叶绿体发育机制提供了全面的信息。航天技术为作物诱变育种提供了一种潜在的手段。
Chloroplast development is an integral part of plant survival and growth, and occurs in parallel with chlorophyll biosynthesis. However, little is known about the mechanisms underlying chloroplast development in hexaploid wheat. Here, we obtained a spaceflight-induced wheat albino mutant mta. Chloroplast ultra-structural observation showed that chloroplasts of mta exhibit abnormal morphology and distribution compared to wild type. Photosynthetic pigments content was also significantly decreased in mta. Transcriptome and chloroplast proteome profiling of mta and wild type were done to identify differentially expressed genes (DEGs) and proteins (DEPs), respectively. In total 4,588 DEGs including 1,980 up- and 2,608 down-regulated, and 48 chloroplast DEPs including 15 up- and 33 down-regulated were identified in mta. Classification of DEGs revealed that most were involved in chloroplast development, chlorophyll biosynthesis, or photosynthesis. Besides, transcription factors such as PIF3, GLK and MYB which might participate in those pathways were also identified. The correlation analysis between DEGs and DEPs revealed that the transcript-to-protein in abundance was functioned into photosynthesis and chloroplast relevant groups. Real time qPCR analysis validated that the expression level of genes encoding photosynthetic proteins was significantly decreased in mta. Together, our results suggest that the molecular mechanism for albino leaf color formation in mta is a thoroughly regulated and complicated process. The combined analysis of transcriptome and proteome afford comprehensive information for further research on chloroplast development mechanism in wheat. And spaceflight provides a potential means for mutagenesis in crop breeding.