Activating Autophagy by Aerobic Exercise in Mice.
Activating Autophagy by Aerobic Exercise in Mice.
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DOI：
10.3791/55099
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发表时间：
2017-02-03
期刊：
影响因子：
--
通讯作者：
He C
中科院分区：
文献类型：
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作者：
Rocchi A;He C
自噬是细胞内环境稳定、功能和分化所必需的溶酶体降解途径。在应激条件下，自噬被诱导并靶向各种货物，如大量细胞质、受损的细胞器和错误折叠的蛋白质，以在溶酶体中降解。产生的营养分子被回收回细胞质，用于新的蛋白质合成和ATP生产。自噬的上调对许多疾病的发病机制具有有益作用，并且已经报道了激活自噬的药理学和生理学策略。有氧运动是近年来发现的一种有效的自噬诱导剂，可诱导小鼠多个器官的自噬，包括肌肉、肝脏、心脏和大脑。在这里，我们展示了通过强迫跑步机运动或自愿轮跑来诱导体内自噬的程序。我们还展示了显微镜和生物化学方法来定量分析小鼠组织中的自噬水平，使用标记蛋白LC 3和p62，它们与自噬体沿着运输到溶酶体并在溶酶体中降解。
Autophagy is a lysosomal degradation pathway essential for cell homeostasis, function and differentiation. Under stress conditions, autophagy is induced and targets various cargos, such as bulk cytosol, damaged organelles and misfolded proteins, for degradation in lysosomes. Resulting nutrient molecules are recycled back to the cytosol for new protein synthesis and ATP production. Upregulation of autophagy has beneficial effects against the pathogenesis of many diseases, and pharmacological and physiological strategies to activate autophagy have been reported. Aerobic exercise is recently identified as an efficient autophagy inducer in multiple organs in mice, including muscle, liver, heart and brain. Here we show procedures to induce autophagy in vivo by either forced treadmill exercise or voluntary wheel running. We also demonstrate microscopic and biochemical methods to quantitatively analyze autophagy levels in mouse tissues, using the marker proteins LC3 and p62 that are transported to and degraded in lysosomes along with autophagosomes.