Rate of decrease of PO2 from an arteriole with arrested flow.
Rate of decrease of PO2 from an arteriole with arrested flow.
复制标题
血流停止的小动脉 PO2 下降率。
DOI：
10.1007/0-387-26206-7_34
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发表时间：
2005
影响因子：
--
通讯作者：
Schleicher,WilliamF
中科院分区：
文献类型：
--
作者：
Pittman,RolandN;Golub,AleksanderS;Schleicher,WilliamF
当流向某个区域的血流停止时，静止血液中所含的氧气量以取决于周围组织的氧气利用率的速率减少。我们用磷光猝灭显微镜测量雄性Sprague-Dawley大鼠肠系膜小动脉PO 2。通过使用连接到显微镜物镜的可充气萨兰袋闭塞微血管来快速停止流动（< 1秒）。闭塞后PO 2的下降率计算出血管腔的初始氧流量为8.0 × 10− 7 ml O2 cm − 2 sec −1。通过假设管腔中的所有氧气以初始速率被壁消耗来计算小动脉壁的氧气消耗的上限。这个数值为2.5 × 10− 3 ml O2 cm −3sec− 1，由于忽略了附近实质细胞的耗氧量，因此被高估了。计算出的壁的最大耗氧量比先前报道的大鼠肠系膜小动脉的最大耗氧量（6.5 × 10− 2 ml O2 cm −3sec−1）小一个数量级以上。我们的结论是，氧消耗的小动脉壁是类似于以前的其他血管组织的值。
When flow to a region is arrested, the amount of oxygen contained within the stationary blood decreases at a rate dependent on the oxygen utilization of the surrounding tissue. We used phosphorescence quenching microscopy to measure arteriolar PO2in the mesentery of male Sprague-Dawley rats. Flow was quickly stopped (< 1 s) by occluding the microvessels using an inflatable Saran bag attached to the microscope objective. The rate of decline in PO2following occlusion yielded a calculated initial flux of oxygen out of the vessel lumen of 8.0 × 10−7ml O2cm−2sec−1. An upper limit on the oxygen consumption of the arteriolar wall was calculated by assuming that all of the oxygen in the lumen was consumed by the wall at the initial rate. This value was 2.5 × 10−3ml O2cm−3sec−1and is an overestimate since the oxygen consumption of the nearby parenchymal cells was neglected. The calculated maximum oxygen consumption of the wall is more than an order of magnitude smaller than that reported previously for arterioles in the rat mesentery (6.5 × 10−2ml O2cm−3sec−1). We conclude that oxygen consumption of the arteriolar wall is similar to previous values for other vascular tissues.