Development of Neutralization Assay Using an eGFP Chikungunya Virus.
Development of Neutralization Assay Using an eGFP Chikungunya Virus.
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使用 eGFP 基孔肯雅病毒开发中和测定法
DOI：
10.3390/v8070181
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发表时间：
2016-06-28
期刊：
影响因子：
--
通讯作者：
Zhang B
中科院分区：
文献类型：
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作者：
Deng CL;Liu SQ;Zhou DG;Xu LL;Li XD;Zhang PT;Li PH;Ye HQ;Wei HP;Yuan ZM;Qin CF;Zhang B
基孔肯雅病毒(CHIKV)是甲型病毒属的成员，是一种重要的人类新/复现病原体。目前，还没有有效的抗病毒药物或疫苗来对抗CHIKV感染。在这里，我们构建了CHIKV的感染性克隆和EGFP报告基因CHIKV(EGFP-CHIKV)，其分离株(归属于亚洲血统)来自CHIKV感染患者。EGFP-CHIKV报告病毒可以通过EGFP表达水平直接观察病毒复制。利用已知的CHIKV抑制剂利巴韦林，我们证实了EGFP-CHIKV报告病毒可以用于识别针对CHIKV的抑制剂。重要的是，我们发展了一种新的、可靠的基于EGFP-CHIKV报告病毒的中和试验，可以用于快速筛选针对CHIKV的中和抗体。
Chikungunya virus (CHIKV), a member of the Alphavirus genus, is an important human emerging/re-emerging pathogen. Currently, there are no effective antiviral drugs or vaccines against CHIKV infection. Herein, we construct an infectious clone of CHIKV and an eGFP reporter CHIKV (eGFP-CHIKV) with an isolated strain (assigned to Asian lineage) from CHIKV-infected patients. The eGFP-CHIKV reporter virus allows for direct visualization of viral replication through the levels of eGFP expression. Using a known CHIKV inhibitor, ribavirin, we confirmed that the eGFP-CHIKV reporter virus could be used to identify inhibitors against CHIKV. Importantly, we developed a novel and reliable eGFP-CHIKV reporter virus-based neutralization assay that could be used for rapid screening neutralizing antibodies against CHIKV.