Cryopreservation of spermatozoa in alginic acid capsules
Cryopreservation of spermatozoa in alginic acid capsules
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DOI：
10.1016/j.fertnstert.2005.06.049
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发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
Beier, HM
中科院分区：
文献类型：
--
作者：
Herrler, A;Eisner, S;Beier, HM
目的：开发一种在聚合藻酸液滴中冷冻少量精子的方法，该方法解冻后可液化以回收精子。设计：前瞻性临床研究。设置：医学院，RWTH亚琛，亚琛，德国。患者：无。干预：验证牛精子包封方法;在藻酸胶囊中冷冻保存人类精子。主要结果测量：我们通过测试影响冷冻程序的不同参数（如海藻酸浓度、液滴大小、聚合时间和培养基）来优化冷冻保存方法。通过7.3mg/mL海藻酸形成10 μ L液滴进行包封，聚合30秒，并在柠檬酸钠中液化2.5分钟。人精子的冷冻保存。与标准方法相比，两种方法冷冻后精子活力降低18.3%，而不动精子活力提高19.9%。将进行进一步的研究，以减少海藻酸微囊化后不动但有活力的精子的数量。
Objective: To develop a method of freezing small amounts of spermatozoa in polymerized alginic acid drops, which can be liquified after thawing for recovery of the spermatozoa.Design: Prospective clinical study.Setting: Medical School, RWTH Aachen, Aachen Germany.Patient(s): None.Intervention(s): Validation of the encapsulation method with bovine sperm; cryopreservation of human spermatozoa in alginic capsules.Main Outcome Measure(s): We optimized the cryopreservation method by testing different parameters influencing the freezing procedure, such as concentration of alginic acid, size of drops, time of polymerization, and culture media.Result(s): The final protocol was as follows: encapsulation by 7.3 mg/mL alginic acid forming 10-mu L drops polymerized for 30 seconds and liquefied for 2.5 minutes in sodium citrate. Cryopreservation of human spermatozoa. by this protocol resulted in a decreased motility of 18.3% compared with standard protocols but a 19.9% higher vitality of the immotile spermatozoa.Conclusion(s): No difference in viability of spermatozoa after both sperm-freezing procedures could be observed. Further investigation will be undertaken to reduce the amount of immotile but viable sperm after microencapsulation in alginic acid.