Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli
Hydrogen peroxide increases the activities of soxRS regulon enzymes and the levels of oxidized proteins and lipids in Escherichia coli
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DOI：
10.1016/j.cellbi.2005.08.002
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发表时间：
2005-11-01
影响因子：
3.9
通讯作者：
Lushchak, V
中科院分区：
文献类型：
--
作者：
Semchyshyn, H;Bagnyukova, T;Lushchak, V
通过监测氧化损伤产物、羰基蛋白和硫代巴比妥酸反应物质 (TBARS) 的积累以及选定的抗氧化酶的活性，评估过氧化氢处理对大肠杆菌 KS400 和 AB 1157 细胞的影响。 H2O2 处理刺激 KS400 细胞中 TBARS 和羰基蛋白水平以剂量和时间依赖性方式增加。 H2O2 处理后 TBARS 的积累变化更大； 5μM H2O2 下 TBARS 含量显着增加，而 100μM H2O2 下羰基蛋白含量显着增加。类似地，用20μM过氧化氢处理不同时间长度导致TBARS积累在20分钟达到峰值，而羰基蛋白水平仅在60分钟后显着升高。在AB 1157细胞中，用20μM过氧化氢处理20分钟导致羰基蛋白和TBARS水平的强烈增加。这种处理还引发了两种菌株中 oxyR 调节子酶（过氧化氢酶、过氧化物酶和谷胱甘肽还原酶）活性的增加。在 AB1157 菌株中，H2O2 暴露还使 soxRS 调节子的两种酶（超氧化物歧化酶和葡萄糖-6-磷酸脱氢酶）的活性增加了 50 - 60%。数据显示了脂质与蛋白质对 H2O2 引起的氧化损伤的不同变异性，以及损伤产物积累和抗氧化酶对 H2O2 应激反应的菌株特异性差异。 (c) 2005 年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
The effects of hydrogen peroxide treatments on Escherichia coli KS400 and AB 1157 cells were assessed by monitoring the accumulation of oxidative damage products, carbonyl proteins and thiobarbituric acid-reactive substances (TBARS), as well as the activities of selected antioxidant enzymes. H2O2 treatment stimulated increases in both TBARS and carbonyl protein levels in dose- and time-dependent manners in KS400 cells. The accumulation of TBARS was much more variable with H2O2 treatment; TBARS content was significantly increased in response to 5 mu M H2O2, whereas a significant increase in carbonyl protein content occurred at 100 mu M H2O2. Similarly, treatment with 20 mu M hydrogen peroxide for different lengths of time resulted in peak TBARS accumulation by 20 min, whereas carbonyl protein levels were significantly elevated only after 60 min. In AB 1157 cells, treatment with 20 mu M hydrogen peroxide for 20 min led to strong increases in both carbonyl protein and TBARS levels. This treatment also triggered increased activities of enzymes of the oxyR regulon (catalase, peroxidase, and glutathione reductase) in both strains. In the AB1157 strain, H2O2 exposure also increased the activities of two enzymes of the soxRS regulon (superoxide dismutase and glucose-6-phosphate dehydrogenase) by 50 - 60%. The data show differential variability of lipids versus proteins to oxidative damage induced by H2O2 as well as strain-specific differences in the accumulation of damage products and the responses by antioxidant enzymes to H2O2 stress. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.