Selective Detection and Quantification of Modified DNA with Solid-State Nanopores
Selective Detection and Quantification of Modified DNA with Solid-State Nanopores
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DOI：
10.1021/nl501340d
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发表时间：
2014-10-01
期刊：
影响因子：
10.8
通讯作者：
Hall, Adam R.
中科院分区：
文献类型：
--
作者：
Carlsen, Autumn T.;Zahid, Osama K.;Hall, Adam R.
我们展示了一种固态纳米孔测定法，用于修饰DNA的明确区分和定量。单个链霉亲和素蛋白被用作含有单个生物素片段的DNA的高亲和力标签。我们确定易位事件的速率直接对应于蛋白质-DNA复合物的相对浓度，并使用我们的方法的选择性，从溶液中未修饰的DNA背景中定量修饰的寡核苷酸。
We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin protein are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events coresponds directly to relative concentration of protein-DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.