Aerosol Jet Printing-Enabled Dual-Function Electrochemical and Colorimetric Biosensor for SARS-CoV-2 Detection.
Aerosol Jet Printing-Enabled Dual-Function Electrochemical and Colorimetric Biosensor for SARS-CoV-2 Detection.
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DOI：
10.1021/acs.analchem.3c01724
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发表时间：
2023-07
影响因子：
7.4
通讯作者：
Li Liu;Zhiheng Xu;A. M. Molina Vargas;S. Dollery;M. Schrlau;D. Cormier;M. O’Connell;G. Tobin;Ke Du
中科院分区：
文献类型：
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作者：
Li Liu;Zhiheng Xu;A. M. Molina Vargas;S. Dollery;M. Schrlau;D. Cormier;M. O’Connell;G. Tobin;Ke Du
以 SARS-CoV-2 RNA 为例，开发了一种支持气溶胶喷射打印的双功能生物传感器，用于敏感检测病原体。 CRISPR-Cas13:guide-RNA 复合物在目标 RNA 存在的情况下被激活，导致含有辣根过氧化物酶 (HRP) 标签的 ssRNA 探针发生附带反式切割。这反过来又催化 HRP 氧化 3,3',5,5'-四甲基联苯胺 (TMB)，导致颜色变化和电化学信号变化。比色和电化学传感方案不需要复杂的目标扩增和探针固定，并且表现出飞摩尔范围内的检测灵敏度。此外，我们的生物传感器具有 5 个数量级的宽动态范围。这种低成本的气溶胶喷墨打印技术可实现无扩增的集成双功能生物传感器平台，该平台在生理温度下运行，专为在资源匮乏的环境或医院中进行简单、快速和准确的护理点 (POC) 诊断而设计。
An aerosol jet printing-enabled dual-function biosensor for the sensitive detection of pathogens using SARS-CoV-2 RNA as an example has been developed. A CRISPR-Cas13:guide-RNA complex is activated in the presence of a target RNA, leading to the collateral trans-cleavage of ssRNA probes that contain a horseradish peroxidase (HRP) tag. This, in turn, catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HRP, resulting in a color change and electrochemical signal change. The colorimetric and electrochemical sensing protocol does not require complicated target amplification and probe immobilization and exhibits a detection sensitivity in the femtomolar range. Additionally, our biosensor demonstrates a wide dynamic range of 5 orders of magnitude. This low-cost aerosol inkjet printing technique allows for an amplification-free and integrated dual-function biosensor platform, which operates at physiological temperature and is designed for simple, rapid, and accurate point-of-care (POC) diagnostics in either low-resource settings or hospitals.